Apolipoprotein A1 antibody


Human apolipoprotein A1 | 100% |
Human apolipoprotein B | <3% |
Human albumin | <1% |
Apolipoprotein A1 is a single polypeptide with a molecular weight of 28 kDa. It is the major protein component of high density lipoprotein particles and functions in preventing the accumulation of cholesterol loaded macrophages which deposit on the arterial wall as foam cells.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rabbit - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Use sterile DI water
- Preparation
- Purified IgG prepared by affinity chromatography
- Preparation
- Purified IgG prepared by affinity chromatography on immobilized human apolipoprotein A1
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
50% Glycerol 0.5% Bovine Serum Albumin 0.01% Thiomersal - Preservative Stabilisers
- Contains mannitol, dextran & salts
- Immunogen
- Purified human apolipoprotein A1
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4°C.
After reconstitution store at -20°C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. - Guarantee
- 12 months from date of despatch
- Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- UniProt
- P02647
- Entrez Gene
- APOA1
- GO Terms
- GO:0005886 plasma membrane
- GO:0001540 beta-amyloid binding
- GO:0002576 platelet degranulation
- GO:0002740 negative regulation of cytokine secretion involved in immune response
- GO:0005543 phospholipid binding
- GO:0005788 endoplasmic reticulum lumen
- GO:0030141 stored secretory granule
- GO:0030139 endocytic vesicle
- GO:0006656 phosphatidylcholine biosynthetic process
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0042802 identical protein binding
- GO:0010873 positive regulation of cholesterol esterification
- GO:0010903 negative regulation of very-low-density lipoprotein particle remodeling
- GO:0015485 cholesterol binding
- GO:0050821 protein stabilization
- GO:0017127 cholesterol transporter activity
- GO:0019899 enzyme binding
- GO:0030168 platelet activation
- GO:0032488 Cdc42 protein signal transduction
- GO:0033344 cholesterol efflux
- GO:0033700 phospholipid efflux
- GO:0034191 apolipoprotein A-I receptor binding
- GO:0034361 very-low-density lipoprotein particle
- GO:0034366 spherical high-density lipoprotein particle
- GO:0034375 high-density lipoprotein particle remodeling
- GO:0034380 high-density lipoprotein particle assembly
- GO:0034384 high-density lipoprotein particle clearance
- GO:0042632 cholesterol homeostasis
- GO:0043691 reverse cholesterol transport
- GO:0050713 negative regulation of interleukin-1 beta secretion
- GO:0051345 positive regulation of hydrolase activity
- GO:0060228 phosphatidylcholine-sterol O-acyltransferase activator activity
- GO:0070508 cholesterol import
- GO:0070653 high-density lipoprotein particle receptor binding
- Regulatory
- For research purposes only
Applications of Apolipoprotein A1 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/1,000 | 1/5,000 | |
Western Blotting | |||
ELISA | 1/5000 | 1/20000 | |
Western Blotting |
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml |
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|
Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F IF | 0.1 mg |
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|
Donkey anti Sheep/Goat IgG:HRP | STAR88P | C E P WB | 1 ml |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
TMB Signal+ | BUF054A | E | 100 ml | ||
TMB Core | BUF056A | E | 100 ml | ||
TMB Core+ | BUF062A | E | 100 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml |
Product Specific References
References for Apolipoprotein A1 antibody
-
Thomson, R. et al. (2009) Hydrodynamic gene delivery of baboon trypanosome lytic factor eliminates both animal and human-infective African trypanosomes.
Proc Natl Acad Sci U S A. 106: 19509-14. -
Niimi, M. et al. (2016) ApoE knockout rabbits: A novel model for the study of human hyperlipidemia.
Atherosclerosis. 245: 187-93. -
Lu, R. et al. (2018) Spontaneous severe hypercholesterolemia and atherosclerosis lesions in rabbits with deficiency of low-density lipoprotein receptor (LDLR) on exon 7.
EBioMedicine. Sep 19 [Epub ahead of print]. -
Yuan, T. et al. (2019) Generation of hyperlipidemic rabbit models using multiple sgRNAs targeted CRISPR/Cas9 gene editing system.
Lipids Health Dis. 18 (1): 69. -
Zhang, T. et al. (2020) Hyperhomocysteinemia and dyslipidemia in point mutation G307S of cystathionine β-synthase-deficient rabbit generated using CRISPR/Cas9.
Lipids Health Dis. 19 (1): 224.
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