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Apolipoprotein A1 antibody

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Anti Human Apolipoprotein A1 Antibody gallery image 1
Published customer image:
Sheep anti Human Apolipoprotein A1 antibody (0650-0180) used for the evaluation of ApoA1 levels in rabbit plasma samples by western blotting.
Image caption:
Analysis of plasma lipids and lipoproteins. (a) Agarose gel electrophoresis of plasma lipoproteins. 4 μl of plasma was loaded in each well and fractionated on a 1% agarose gel and stained with Fat Red 7B for neutral lipids. Lipoprotein migration positions are indicated by arrows. (b) Analysis of plasma apolipoproteins by Western blotting. Plasma samples (0.5μl) were fractionated on 10% SDS-PAGE and transferred to a cellulose membrane probed with Abs against apoB, apoE and apoAI as described in the Materials and Methods section. (c) Plasma levels of total cholesterol (TC), triglycerides (TG), LDL-cholesterol (LDL-C) and HDL-cholesterol (HDL-C)

From: Yuan T, Zhong Y, Wang Y, Zhang T, Lu R, Zhou M, Lu Y, Yan K, Chen Y, Hu Z, Liang J, Fan J, Cheng Y.
Generation of hyperlipidemic rabbit models using multiple sgRNAs targeted CRISPR/Cas9 gene editing system.
Lipids Health Dis. 2019;18(1):69.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Sheep anti Human Apolipoprotein A1:HRP

Sheep anti Human Apolipoprotein A1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
0650-0190
Datasheet
E WB
MSDS
0.2 mg

0650-0180
Datasheet
E WB
MSDS
0.5 mg

Sheep anti Human Apolipoprotein A1 antibody reacts with human apolipoprotein A1 and does not react with other human plasma proteins. In ELISA the following reactivities are observed:

Human apolipoprotein A1100%
Human apolipoprotein B<3%
Human albumin<1%


Apolipoprotein A1 is a single polypeptide with a molecular weight of 28 kDa. It is the major protein component of high density lipoprotein particles and functions in preventing the accumulation of cholesterol loaded macrophages which deposit on the arterial wall as foam cells.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - lyophilised
Reconstitution
Use sterile DI water
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography on immobilized human apolipoprotein A1
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
50%Glycerol
0.5%Bovine Serum Albumin
0.01%Thiomersal
Preservative Stabilisers
Contains mannitol, dextran & salts
Immunogen
Purified human apolipoprotein A1
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4°C.
After reconstitution store at -20°C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Guarantee
12 months from date of despatch
Guarantee
Guaranteed until date of expiry. Please see product label.

More Information

UniProt
P02647
Entrez Gene
APOA1
GO Terms
GO:0005886 plasma membrane
GO:0001540 beta-amyloid binding
GO:0002576 platelet degranulation
GO:0002740 negative regulation of cytokine secretion involved in immune response
GO:0005543 phospholipid binding
GO:0005788 endoplasmic reticulum lumen
GO:0030141 stored secretory granule
GO:0030139 endocytic vesicle
GO:0006656 phosphatidylcholine biosynthetic process
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0042802 identical protein binding
GO:0010873 positive regulation of cholesterol esterification
GO:0010903 negative regulation of very-low-density lipoprotein particle remodeling
GO:0015485 cholesterol binding
GO:0050821 protein stabilization
GO:0017127 cholesterol transporter activity
GO:0019899 enzyme binding
GO:0030168 platelet activation
GO:0032488 Cdc42 protein signal transduction
GO:0033344 cholesterol efflux
GO:0033700 phospholipid efflux
GO:0034191 apolipoprotein A-I receptor binding
GO:0034361 very-low-density lipoprotein particle
GO:0034366 spherical high-density lipoprotein particle
GO:0034375 high-density lipoprotein particle remodeling
GO:0034380 high-density lipoprotein particle assembly
GO:0034384 high-density lipoprotein particle clearance
GO:0042632 cholesterol homeostasis
GO:0043691 reverse cholesterol transport
GO:0050713 negative regulation of interleukin-1 beta secretion
GO:0051345 positive regulation of hydrolase activity
GO:0060228 phosphatidylcholine-sterol O-acyltransferase activator activity
GO:0070508 cholesterol import
GO:0070653 high-density lipoprotein particle receptor binding
Regulatory
For research purposes only

Applications of Apolipoprotein A1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1,000 1/5,000
Western Blotting
ELISA 1/5000 1/20000
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
ELISA
0650-0190 can be used in a sandwich ELISA as a detection antibody together with 0650-0050 as the capture antibody.
ELISA
This antibody can be used in a sandwich ELISA with 0650-0050 as the capture antibody.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 C E F WB 1.5 ml loader
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA F IF 0.1 mg loader
Donkey anti Sheep/Goat IgG:FITC STAR88F C 1 mg loader
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
TMB Signal+ BUF054A E 100 ml
TMB Core BUF056A E 100 ml
TMB Core+ BUF062A E 100 ml

Application Based External Images

Western Blotting

Product Specific References

References for Apolipoprotein A1 antibody

  1. Thomson, R. et al. (2009) Hydrodynamic gene delivery of baboon trypanosome lytic factor eliminates both animal and human-infective African trypanosomes.
    Proc Natl Acad Sci U S A. 106: 19509-14.
  2. Niimi, M. et al. (2016) ApoE knockout rabbits: A novel model for the study of human hyperlipidemia.
    Atherosclerosis. 245: 187-93.
  3. Lu, R. et al. (2018) Spontaneous severe hypercholesterolemia and atherosclerosis lesions in rabbits with deficiency of low-density lipoprotein receptor (LDLR) on exon 7.
    EBioMedicine. Sep 19 [Epub ahead of print].
  4. Yuan, T. et al. (2019) Generation of hyperlipidemic rabbit models using multiple sgRNAs targeted CRISPR/Cas9 gene editing system.
    Lipids Health Dis. 18 (1): 69.
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