Apolipoprotein A1 antibody
Sheep anti Human Apolipoprotein A1 antibody (0650-0180) used for the evaluation of ApoA1 levels in rabbit plasma samples by western blotting.
Image caption:
Analysis of plasma lipids and lipoproteins. (a) Agarose gel electrophoresis of plasma lipoproteins. 4 μl of plasma was loaded in each well and fractionated on a 1% agarose gel and stained with Fat Red 7B for neutral lipids. Lipoprotein migration positions are indicated by arrows. (b) Analysis of plasma apolipoproteins by Western blotting. Plasma samples (0.5μl) were fractionated on 10% SDS-PAGE and transferred to a cellulose membrane probed with Abs against apoB, apoE and apoAI as described in the Materials and Methods section. (c) Plasma levels of total cholesterol (TC), triglycerides (TG), LDL-cholesterol (LDL-C) and HDL-cholesterol (HDL-C)
From: Yuan T, Zhong Y, Wang Y, Zhang T, Lu R, Zhou M, Lu Y, Yan K, Chen Y, Hu Z, Liang J, Fan J, Cheng Y.
Generation of hyperlipidemic rabbit models using multiple sgRNAs targeted CRISPR/Cas9 gene editing system.
Lipids Health Dis. 2019;18(1):69.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Sheep anti Human apolipoprotein A1 antibody (0650-0180) used for the evaluation of apolipoprotein A1 in rabbit plasma samples by western blotting.
Image caption:
CBS-KO rabbits are hyperlipidemic.
Immunoblot showing levels of plasma apolipoproteins in the indicated groups. WT-NC: wild type rabbits fed normal chow. WT-SB: wild type rabbits fed normal chow supplemented with the vitamin B and betaine complex. C2♀, C8♀: CBS-KO rabbits on a normal chow diet. C9♀, C14♂, C15♀, C16♂: CBS-KO rabbits fed normal chow supplemented with the vitamin B and betaine complex♀
From: Zhang, T., Lu, R., Chen, Y. et al.
Hyperhomocysteinemia and dyslipidemia in point mutation G307S of cystathionine β-synthase-deficient rabbit generated using CRISPR/Cas9.
Lipids Health Dis 19, 224.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Sheep anti Human Apolipoprotein A1 antibody reacts with human apolipoprotein A1 and does not react with other human plasma proteins. In ELISA the following reactivities are observed:
Apolipoprotein A1 is a single polypeptide with a molecular weight of 28 kDa. It is the major protein component of high density lipoprotein particles and functions in preventing the accumulation of cholesterol loaded macrophages which deposit on the arterial wall as foam cells.
| Human apolipoprotein A1 | 100% |
| Human apolipoprotein B | <3% |
| Human albumin | <1% |
Apolipoprotein A1 is a single polypeptide with a molecular weight of 28 kDa. It is the major protein component of high density lipoprotein particles and functions in preventing the accumulation of cholesterol loaded macrophages which deposit on the arterial wall as foam cells.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rabbit - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Use sterile DI water
- Preparation
- Purified IgG prepared by affinity chromatography
- Preparation
- Purified IgG prepared by affinity chromatography on immobilized human apolipoprotein A1
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- 0.1M Sodium Chloride
0.01M Sodium Phosphate
0.01M Sodium Borate - Preservative Stabilisers
50% Glycerol 0.5% Bovine Serum Albumin 0.01% Thiomersal - Preservative Stabilisers
- 1% Mannitol
1% Dextran - Immunogen
- Purified human apolipoprotein A1
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- Prior to reconstitution store at +4°C.
After reconstitution store at -20°C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. - Guarantee
- 12 months from date of despatch
- Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- UniProt
- P02647
- Entrez Gene
- APOA1
- GO Terms
- GO:0005886 plasma membrane
- GO:0001540 beta-amyloid binding
- GO:0002576 platelet degranulation
- GO:0002740 negative regulation of cytokine secretion involved in immune response
- GO:0005543 phospholipid binding
- GO:0005788 endoplasmic reticulum lumen
- GO:0030141 stored secretory granule
- GO:0030139 endocytic vesicle
- GO:0006656 phosphatidylcholine biosynthetic process
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0042802 identical protein binding
- GO:0010873 positive regulation of cholesterol esterification
- GO:0010903 negative regulation of very-low-density lipoprotein particle remodeling
- GO:0015485 cholesterol binding
- GO:0050821 protein stabilization
- GO:0017127 cholesterol transporter activity
- GO:0019899 enzyme binding
- GO:0030168 platelet activation
- GO:0032488 Cdc42 protein signal transduction
- GO:0033344 cholesterol efflux
- GO:0033700 phospholipid efflux
- GO:0034191 apolipoprotein A-I receptor binding
- GO:0034361 very-low-density lipoprotein particle
- GO:0034366 spherical high-density lipoprotein particle
- GO:0034375 high-density lipoprotein particle remodeling
- GO:0034380 high-density lipoprotein particle assembly
- GO:0034384 high-density lipoprotein particle clearance
- GO:0042632 cholesterol homeostasis
- GO:0043691 reverse cholesterol transport
- GO:0050713 negative regulation of interleukin-1 beta secretion
- GO:0051345 positive regulation of hydrolase activity
- GO:0060228 phosphatidylcholine-sterol O-acyltransferase activator activity
- GO:0070508 cholesterol import
- GO:0070653 high-density lipoprotein particle receptor binding
- Regulatory
- For research purposes only
Applications of Apolipoprotein A1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/1,000 | 1/5,000 | |
| Western Blotting | |||
| ELISA | 1/5000 | 1/20000 | |
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml |
|
|
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F IF | 0.1 mg |
|
|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | C E P WB | 1 ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for Apolipoprotein A1 antibody
-
Thomson, R. et al. (2009) Hydrodynamic gene delivery of baboon trypanosome lytic factor eliminates both animal and human-infective African trypanosomes.
Proc Natl Acad Sci U S A. 106: 19509-14. -
Niimi, M. et al. (2016) ApoE knockout rabbits: A novel model for the study of human hyperlipidemia.
Atherosclerosis. 245: 187-93. -
Lu, R. et al. (2018) Spontaneous severe hypercholesterolemia and atherosclerosis lesions in rabbits with deficiency of low-density lipoprotein receptor (LDLR) on exon 7.
EBioMedicine. Sep 19 [Epub ahead of print]. -
Yuan, T. et al. (2019) Generation of hyperlipidemic rabbit models using multiple sgRNAs targeted CRISPR/Cas9 gene editing system.
Lipids Health Dis. 18 (1): 69. -
Zhang, T. et al. (2020) Hyperhomocysteinemia and dyslipidemia in point mutation G307S of cystathionine β-synthase-deficient rabbit generated using CRISPR/Cas9.
Lipids Health Dis. 19 (1): 224.
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