Rabbit anti APOBEC3B antibody
recognizes DNA dC->dU-editing enzyme APOBEC3B, also known as phorbolin-1-related protein and phorbolin-2/3. It is a member of the APOBEC (apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like) family of cytidine deaminases (Kuong and Loeb 2013
). APOBEC family members function as a part of the innate immune system inhibiting retroviruses by deamination of cytosine in nascent retroviral cDNA (Harris and Liddament 2004
). APOBEC3 proteins are divided into two groups: APOBEC3A, APOBEC3C, and APOBEC3H that contain one zinc-dependent cytidine deaminase domain and APOBEC3B, APOBEC3D, APOBEC3F, and APOBEC3G that contain two zinc-dependent cytidine deaminase domains (LaRue et al. 2008
). APOBEC3B is overexpressed in a wide spectrum of human cancers (Zou et al. 2017
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to APOBEC3B was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human APOBEC3B
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
zinc ion binding
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of APOBEC3B antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti APOBEC3B antibody detects a band of approximately 46 kDa in HeLa, U251 and MCF7 cell lysates under reducing conditions
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Secondary Antibodies Available
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