APOBEC3B antibody

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Rabbit anti APOBEC3B

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2737
Datasheet
WB
SDS
50 µl loader

Rabbit anti APOBEC3B antibody recognizes DNA dC->dU-editing enzyme APOBEC3B, also known as phorbolin-1-related protein and phorbolin-2/3. It is a member of the APOBEC (apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like) family of cytidine deaminases (Kuong and Loeb 2013). APOBEC family members function as a part of the innate immune system inhibiting retroviruses by deamination of cytosine in nascent retroviral cDNA (Harris and Liddament 2004). APOBEC3 proteins are divided into two groups: APOBEC3A, APOBEC3C, and APOBEC3H that contain one zinc-dependent cytidine deaminase domain and APOBEC3B, APOBEC3D, APOBEC3F, and APOBEC3G that contain two zinc-dependent cytidine deaminase domains (LaRue et al. 2008). APOBEC3B is overexpressed in a wide spectrum of human cancers (Zou et al. 2017).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to APOBEC3B was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human APOBEC3B

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q9UH17
Entrez Gene
APOBEC3B
GO Terms
GO:0003723 RNA binding
GO:0008270 zinc ion binding
GO:0016814 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines
Regulatory
For research purposes only

Applications of APOBEC3B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/200 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti APOBEC3B antibody detects a band of approximately 46 kDa in HeLa, U251 and MCF7 cell lysates under reducing conditions

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg