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APEX1 antibody

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KO Validated
Anti APEX1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1 Anti APEX1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2 Anti APEX1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3
Anti APEX1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with APEX1 antibody followed by detection with Goat anti Rabbit HRP conjugated (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 34 second exposure. Arrow points to APEX1 (molecular weight 34 kDa).
Anti APEX1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 2
Western blot analysis of APEX1 knockout HEK293 and wild type HEK293 (WT) whole cell lysates probed with Rabbit anti APEX1 antibody (VPA00743) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000 STAR208P) and visualized on the ChemiDoc MP with a 95 second exposure. Tubulin was detected with hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a 30 second exposure.
Anti APEX1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 3
Normalized signal intensity of APEX1 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Rabbit anti APEX1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
APEX1

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Product Code Applications Pack Size List Price Your Price Qty
VPA00743
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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loader

Rabbit anti Human APEX1 antibody recognizes apurinic/apyrimidinic endodeoxyribonuclease 1 also known as AP endonuclease class I, AP lyase, deoxyribonuclase, protein REF-1 or redox factor-1 .

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein (provided by RefSeq, Jul 2008).

Rabbit anti Human APEX1 antibody detects a band of 34 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti APEX1 antibody recognizes a band of approximately 34 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide directed towards the middle region of human APEX1
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (H/L):HRP

UniProt
P27695
Entrez Gene
APEX1
GO Terms
GO:0005515 protein binding
GO:0003684 damaged DNA binding
GO:0003713 transcription coactivator activity
GO:0003714 transcription corepressor activity
GO:0003723 RNA binding
GO:0003906 DNA-(apurinic or apyrimidinic site) lyase activity
GO:0005739 mitochondrion
GO:0004523 ribonuclease H activity
GO:0004528 phosphodiesterase I activity
GO:0004844 uracil DNA N-glycosylase activity
GO:0005783 endoplasmic reticulum
GO:0046872 metal ion binding
GO:0005840 ribosome
GO:0005730 nucleolus
GO:0005813 centrosome
GO:0016491 oxidoreductase activity
GO:0006284 base-excision repair
GO:0006310 DNA recombination
GO:0008408 3'-5' exonuclease activity
GO:0016607 nuclear speck
GO:0016890 site-specific endodeoxyribonuclease activity, specific for altered base
GO:0031490 chromatin DNA binding
GO:0043488 regulation of mRNA stability
GO:0045739 positive regulation of DNA repair
GO:0045768 positive regulation of anti-apoptosis
GO:0048471 perinuclear region of cytoplasm

VPA00743

170515

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