APC8 antibody

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Anti APC8 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with APC8 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 36 second exposure. Arrow points to APC8 (molecular weight 64 kDa)

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Rabbit anti APC8

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: APC8

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Rabbit anti Human APC8 antibody recognizes the cell division cycle protein 23 homolog, also known as anaphase promoting complex subunit 8 or cyclosome subunit 8.

The protein encoded by CDC23 gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction (provided by RefSeq, Jul 2008).

Rabbit anti Human APC8 antibody detects a band of 64 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Rabbit anti APC8 detects a band of approximately 64 kDa in Jurkat cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid.
Preparation: Rabbit polyclonal antibody purified by affinity chromatography on Protein A.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide
2% Sucrose.
Immunogen: Synthetic peptide encompassing part of the N terminal region of human APC8
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: Q9UJX2
Entrez Gene: CDC23
GO Terms: GO:0000080 G1 phase of mitotic cell cycle; GO:0005829 cytosol; GO:0004842 ubiquitin-protein ligase activity; GO:0005488 binding; GO:0005654 nucleoplasm; GO:0005680 anaphase-promoting complex; GO:0007080 mitotic metaphase plate congression; GO:0007091 mitotic metaphase/anaphase transition; GO:0007094 mitotic cell cycle spindle assembly checkpoint; View More GO Terms; GO:0007096 regulation of exit from mitosis; GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; GO:0051301 cell division; GO:0051436 negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; GO:0051437 positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; GO:0070979 protein K11-linked ubiquitination