APBB1 antibody
Rabbit anti APBB1
Rabbit anti human APBB1 antibody recognizes amyloid beta A4 precursor protein-binding family B member 1, also known as FE65 or adaptor protein FE65a2. This antibody has been extensively validated for western blotting using whole cell lysates.
APBB1 Antibody with Control Lysate
Rabbit anti human APBB1 antibody recognizes amyloid beta A4 precursor protein-binding family B member 1, also known as FE65 or adaptor protein FE65a2. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00349 | WB | 100 µl | ||||
| VPA00349KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human APBB1 antibody recognizes APBB1, also known as FE65, adaptor protein FE65a2 and amyloid beta A4 precursor protein-binding family B member 1.
The protein encoded by the APBB1 gene is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein (APP), transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. This encoded protein could play an important role in the pathogenesis of Alzheimer's disease. It is thought to regulate transcription. Also it is observed to block cell cycle progression by downregulating thymidylate synthase expression. Multiple alternatively spliced transcript variants encoding different isoforms have been described for APBB1 (provided by RefSeq, Mar 2012).
Rabbit anti Human APBB1 antibody detects a band of 107 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The protein encoded by the APBB1 gene is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein (APP), transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. This encoded protein could play an important role in the pathogenesis of Alzheimer's disease. It is thought to regulate transcription. Also it is observed to block cell cycle progression by downregulating thymidylate synthase expression. Multiple alternatively spliced transcript variants encoding different isoforms have been described for APBB1 (provided by RefSeq, Mar 2012).
Rabbit anti Human APBB1 antibody detects a band of 107 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti APBB1 detects a band of approximately 107 kDa in HEK293 cell lysate
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Immunogen
- Synthetic peptide corresponding to the N-terminal region of Human APBB1
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- O00213 Related reagents
- Entrez Gene
- APBB1 Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0001540 beta-amyloid binding
- GO:0003682 chromatin binding
- GO:0042393 histone binding
- GO:0006915 apoptosis
- GO:0007165 signal transduction
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0006350 transcription
- GO:0006974 response to DNA damage stimulus
- GO:0007050 cell cycle arrest
- GO:0007409 axonogenesis
- GO:0008134 transcription factor binding
- GO:0016563 transcription activator activity
- GO:0030027 lamellipodium
- GO:0030308 negative regulation of cell growth
- GO:0030426 growth cone
- GO:0043065 positive regulation of apoptosis
- GO:0043967 histone H4 acetylation
- GO:0045202 synapse
- GO:0045749 negative regulation of S phase of mitotic cell cycle
- GO:0050760 negative regulation of thymidylate synthase biosynthetic process
- GO:0070064 proline-rich region binding
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of APBB1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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