APBB1 antibody

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Rabbit anti APBB1

Rabbit anti human APBB1 antibody recognizes amyloid beta A4 precursor protein-binding family B member 1, also known as FE65 or adaptor protein FE65a2. This antibody has been extensively validated for western blotting using whole cell lysates.

APBB1 Antibody with Control Lysate

Rabbit anti human APBB1 antibody recognizes amyloid beta A4 precursor protein-binding family B member 1, also known as FE65 or adaptor protein FE65a2. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00349 WB 100 µl
VPA00349KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human APBB1 antibody recognizes APBB1, also known as FE65, adaptor protein FE65a2 and amyloid beta A4 precursor protein-binding family B member 1.

The protein encoded by the APBB1 gene is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein (APP), transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. This encoded protein could play an important role in the pathogenesis of Alzheimer's disease. It is thought to regulate transcription. Also it is observed to block cell cycle progression by downregulating thymidylate synthase expression. Multiple alternatively spliced transcript variants encoding different isoforms have been described for APBB1 (provided by RefSeq, Mar 2012).

Rabbit anti Human APBB1 antibody detects a band of 107 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti APBB1 detects a band of approximately 107 kDa in HEK293 cell lysate
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide corresponding to the N-terminal region of Human APBB1
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
O00213 Related reagents
Entrez Gene
APBB1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0001540 beta-amyloid binding
GO:0003682 chromatin binding
GO:0042393 histone binding
GO:0006915 apoptosis
GO:0007165 signal transduction
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0006350 transcription
GO:0006974 response to DNA damage stimulus
GO:0007050 cell cycle arrest
GO:0007409 axonogenesis
GO:0008134 transcription factor binding
GO:0016563 transcription activator activity
GO:0030027 lamellipodium
GO:0030308 negative regulation of cell growth
GO:0030426 growth cone
GO:0043065 positive regulation of apoptosis
GO:0043967 histone H4 acetylation
GO:0045202 synapse
GO:0045749 negative regulation of S phase of mitotic cell cycle
GO:0050760 negative regulation of thymidylate synthase biosynthetic process
GO:0070064 proline-rich region binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of APBB1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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