Annexin V antibody

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Rabbit anti Annexin V

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00077
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human annexin V antibody recognizes human annexin V (ANXA5), a member of the annexin family of membrane binding proteins that share structural properties and biological activities associated with membrane related processes. Annexin V, also known as placental anticoagulant protein 4, has potent anticoagulant properties and is amongst the annexins that have extracellular presence in addition to intracellular localization.

The annexin V protein binds with high affinity to membranes bearing phosphatidylserine (PS) and fluorescently labeled annexin V is commonly used as a marker for apoptotic cells in vitro and in vivo.

Rabbit anti Human annexin V antibody detects ANXA5 as a single band of 32 kDa in a range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.

Product Details

Target Species
Human
Western Blotting
Rabbit anti annexin V detects a band of approximately 32 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Human ANXA5 full-length recombinant protein 6x His tagged.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P08758
Entrez Gene
ANXA5
GO Terms
GO:0005509 calcium ion binding
GO:0007596 blood coagulation
GO:0004859 phospholipase inhibitor activity
GO:0007165 signal transduction
GO:0005544 calcium-dependent phospholipid binding
GO:0005737 cytoplasm
GO:0006916 anti-apoptosis
GO:0050819 negative regulation of coagulation
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Annexin V antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml