Annexin V antibody
Rabbit anti Annexin V
Annexin V antibody recognizes annexin A5, also known as anchorin CII, annexin V, annexin-5, calphobindin I, CBP-I, endonexin II, lipocortin V, placental anticoagulant protein 4, PP4, or VAC-alpha. The antibody has been extensively validated for western blotting using whole cell lysates.
Annexin V Antibody with Control Lysate
Annexin V antibody recognizes annexin A5, also known as anchorin CII, annexin V, annexin-5, calphobindin I, CBP-I, endonexin II, lipocortin V, placental anticoagulant protein 4, PP4, placental anticoagulant protein I, PAP-I, or thromboplastin inhibitor.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00077 | WB | 100 µl | ||||
| VPA00077KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human annexin V antibody recognizes human annexin V (ANXA5), a member of the annexin family of membrane binding proteins that share structural properties and biological activities associated with membrane related processes. Annexin V, also known as placental anticoagulant protein 4, has potent anticoagulant properties and is amongst the annexins that have extracellular presence in addition to intracellular localization.
The annexin V protein binds with high affinity to membranes bearing phosphatidylserine (PS) and fluorescently labeled annexin V is commonly used as a marker for apoptotic cells in vitro and in vivo.
Rabbit anti Human annexin V antibody detects ANXA5 as a single band of 32 kDa in a range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.
The annexin V protein binds with high affinity to membranes bearing phosphatidylserine (PS) and fluorescently labeled annexin V is commonly used as a marker for apoptotic cells in vitro and in vivo.
Rabbit anti Human annexin V antibody detects ANXA5 as a single band of 32 kDa in a range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.
Product Details
- Target Species
- Human
- Western Blotting
- Rabbit anti annexin V detects a band of approximately 32 kDa in HeLa cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Human ANXA5 full-length recombinant protein 6x His tagged.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
More Information
- UniProt
- P08758 Related reagents
- Entrez Gene
- ANXA5 Related reagents
- GO Terms
- GO:0005509 calcium ion binding
- GO:0007596 blood coagulation
- GO:0004859 phospholipase inhibitor activity
- GO:0007165 signal transduction
- GO:0005544 calcium-dependent phospholipid binding
- GO:0005737 cytoplasm
- GO:0006916 anti-apoptosis
- GO:0050819 negative regulation of coagulation
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Annexin V antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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