Annexin II antibody
Rabbit anti Human Annexin II antibody recognizes human Annexin A2, also known as Annexin II (ANXA2), lipocortin 2, Chromobindin-8 or Placental anticoagulant protein IV. Annexin II isa 339 amino acid secreted protein containing 4 annexin repeats. Annexin II is a pleiotropic calcium-dependent phospholipid-binding protein and member of the annexin family, abundantly expressed in a variety of tissues including the brain, spleen, kidney and placenta, which can exist in a monomer, heterodimer or heterotetramer form.
Annexin II acts as an autocrine factor, enhancing the formation of osteoclasts and resorption of bone, and is implicated in both exocytosis and endocytosis. Annexin II is a substrate for both tyrosine and serine/threonine kinases, and is phosphorylated following growth factor stimulation and cellular activation by viral transformation (Brambilla et al. 1991).
Annexin II acts as an autocrine factor, enhancing the formation of osteoclasts and resorption of bone, and is implicated in both exocytosis and endocytosis. Annexin II is a substrate for both tyrosine and serine/threonine kinases, and is phosphorylated following growth factor stimulation and cellular activation by viral transformation (Brambilla et al. 1991).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human ANXA2 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- 6xHis-tagged full-length recombinant human ANXA2 (NP_001002857).
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P07355
- Entrez Gene
- ANXA2
- GO Terms
- GO:0001501 skeletal system development
- GO:0005509 calcium ion binding
- GO:0004859 phospholipase inhibitor activity
- GO:0005544 calcium-dependent phospholipid binding
- GO:0005604 basement membrane
- GO:0005625 soluble fraction
- GO:0008092 cytoskeletal protein binding
- GO:0030496 midbody
- GO:0031340 positive regulation of vesicle fusion
- GO:0042470 melanosome
- Regulatory
- For research purposes only
Applications of Annexin II antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Western Blotting | 1/250 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Moss, S.E. and Morgan, R. O. (2004) The annexins.
Genome Biol. 5: 219-219.8
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