Annexin I antibody

100% Secure


Rabbit anti Human Annexin I

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1921 E P* WB 0.1 mg
Rabbit anti Human Annexin I antibody (AHP1921) recognizes human Annexin I (ANXA1), otherwise known as lipocortin I, a calcium-dependent phospholipid-binding protein and member of the annexin family, preferentially expressed on the cytosolic face of the plasma membrane, which acts as an inhibitor of phospholipase A2, and may exhibit anti-inflammatory activity.

Increased expression of the ANXA1 gene in hairy cell leukaemia, can be used in immunohistochemistry as a highly-sensitive diagnostic marker.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human ANXA1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
6xHis-tagged full-length recombinant human ANXA1 (NP_000691.1).
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

UniProt
P04083 Related reagents
Entrez Gene
ANXA1 Related reagents
GO Terms
GO:0005509 calcium ion binding
GO:0001533 cornified envelope
GO:0030674 protein binding, bridging
GO:0005102 receptor binding
GO:0005576 extracellular region
GO:0005198 structural molecule activity
GO:0005544 calcium-dependent phospholipid binding
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005929 cilium
GO:0006629 lipid metabolic process
GO:0006916 anti-apoptosis
GO:0006928 cellular component movement
GO:0006954 inflammatory response
GO:0007166 cell surface receptor linked signaling pathway
GO:0016323 basolateral plasma membrane
GO:0018149 peptide cross-linking
GO:0019834 phospholipase A2 inhibitor activity
GO:0030216 keratinocyte differentiation
GO:0031340 positive regulation of vesicle fusion
GO:0046632 alpha-beta T cell differentiation
Regulatory
For research purposes only

Applications of Annexin I antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin 1 1/3000
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Colon carcinoma

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Moss, S.E. & Morgan, R.O. (2004) The annexins.
    Genome Biol. 5 (4): 219.