Amyloid Precursor Protein antibody

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Rabbit anti Human Amyloid Precursor Protein (KPI Domain)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP665 IP* P* WB* 0.1 ml
Rabbit anti Human Amyloid Precursor Protein antibody recognizes an epitope in the Kunitz protease inhibitor (KPI) domain of human amyloid precursor protein (APP).

Only the APP-751 and APP-770 isoforms contain a KPI domain, it being absent from the APP-695 isoform that is most abundant in neuronal tissue. However, there is evidence that suggests KPI containing isoforms may be important in the pathogenesis of Alzheimer disease.

Rabbit anti Human Amyloid Precursor Protein antibody recognizes a band of ~115kDa in NTera2/DI cell lysates using western blotting under reducing conditions.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to human APP were raised by repeated immunisations of rabbits with highly purified antigen.
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
Synthetic peptide composed of amino acids 292-312 from the KPI domain of human APP.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P05067 Related reagents
Entrez Gene
APP Related reagents
Regulatory
For research purposes only

Applications of Amyloid Precursor Protein antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 1/400
Immunoprecipitation 2 Neat, 5ul/ml
Western Blotting 3 1/500 1/1000
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin embedded sections. 0.05M Tris pH7.0, followed by reduction and alkylation is recommended (Campbell et al. 1999).
  2. 2 This product requires sample reduction and alkylation prior to immunoprecipitation.
  3. 3 Antigen detection may be enhanced following reduction and alkylation of the sample prior to blocking and antibody incubation.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Alzheimer's disease brain.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Product Specific References

References for Amyloid Precursor Protein antibody

  1. Campbell, E. et al. (1999) Methods to uncover an antibody epitope in the KPI domain of Alzheimer's amyloid precursor protein for immunohistochemistry in human brain.
    J. Neurosci. Methods 93: 133-138.
  2. Jelic, V. et al. (2013) Abnormal Platelet Amyloid-β Protein Precursor (AβPP) Metabolism in Alzheimer's Disease: Identification and Characterization of a New AβPP Isoform as Potential Biomarker
    J Alzheimers Dis. 35: 285-95.