AMPK Gamma 1 antibody

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Rabbit anti AMPK Gamma 1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00425
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human AMPK gamma 1 antibody recognizes AMPK gamma 1 also known as 5'-AMP-activated protein kinase subunit gamma-1, PRKAG1 or AMPK gamma-1 chain.

The protein encoded by PRKAG1 is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed (provided by RefSeq, Jul 2008).

Rabbit anti Human AMPK gamma 1 antibody detects a band of 35 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti AMPK gamma 1 detects a band of approximately 35 kDa in Jurkat cell lysates.
Product Form
Purified IgG - liquid.
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide corresponding to amino acid 1-30 of human AMPK gamma 1

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P54619
Entrez Gene
PRKAG1
GO Terms
GO:0005829 cytosol
GO:0004691 cAMP-dependent protein kinase activity
GO:0006110 regulation of glycolysis
GO:0006468 protein phosphorylation
GO:0006633 fatty acid biosynthetic process
GO:0007050 cell cycle arrest
GO:0007283 spermatogenesis
GO:0008286 insulin receptor signaling pathway
GO:0008603 cAMP-dependent protein kinase regulator activity
GO:0019901 protein kinase binding
GO:0045860 positive regulation of protein kinase activity
GO:0046320 regulation of fatty acid oxidation
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of AMPK Gamma 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml