AMPK Gamma 1 antibody

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Anti AMPK Gamma 1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with AMPK gamma 1 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG antibody (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 119 second exposure. Arrow points to AMPK gamma 1 (molecular weight 35 kDa).

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Rabbit anti AMPK Gamma 1

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: AMPK Gamma 1

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Rabbit anti Human AMPK gamma 1 antibody recognizes AMPK gamma 1 also known as 5'-AMP-activated protein kinase subunit gamma-1, PRKAG1 or AMPK gamma-1 chain.

The protein encoded by PRKAG1 is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed (provided by RefSeq, Jul 2008).

Rabbit anti Human AMPK gamma 1 antibody detects a band of 35 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti AMPK gamma 1 detects a band of approximately 35 kDa in Jurkat cell lysates.
Product Form: Purified IgG - liquid.
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: KLH conjugated synthetic peptide corresponding to amino acid 1-30 of human AMPK gamma 1
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P54619
Entrez Gene: PRKAG1
GO Terms: GO:0005829 cytosol; GO:0004691 cAMP-dependent protein kinase activity; GO:0006110 regulation of glycolysis; GO:0006468 protein phosphorylation; GO:0006633 fatty acid biosynthetic process; GO:0007050 cell cycle arrest; GO:0007283 spermatogenesis; GO:0008286 insulin receptor signaling pathway; GO:0008603 cAMP-dependent protein kinase regulator activity; View More GO Terms; GO:0019901 protein kinase binding; GO:0045860 positive regulation of protein kinase activity; GO:0046320 regulation of fatty acid oxidation