AMPK Alpha 1 antibody
Rabbit anti AMPK alpha 1 antibody recognizes 5’-AMP-activated protein kinase catalytic subunit alpha-1 (AMPK alpha 1), also known as AMPK1, AMPK subunit alpha-1 or PRKAA1. AMPK alpha 1 is a member of the serine/threonine protein kinase family. AMPK alpha 1 is the catalytic subunit of 5'-prime-AMP-activated protein kinase (AMPK), and is involved in the regulation of cellular energy homeostasis.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to AMPK alpha 1 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Recombinant human AMPK alpha 1
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q13131
- Entrez Gene
- PRKAA1
- GO Terms
- GO:0000187 activation of MAPK activity
- GO:0005515 protein binding
- GO:0001666 response to hypoxia
- GO:0005829 cytosol
- GO:0004691 cAMP-dependent protein kinase activity
- GO:0046872 metal ion binding
- GO:0005524 ATP binding
- GO:0006633 fatty acid biosynthetic process
- GO:0006695 cholesterol biosynthetic process
- GO:0007050 cell cycle arrest
- GO:0008286 insulin receptor signaling pathway
- GO:0045542 positive regulation of cholesterol biosynthetic process
- GO:0045768 positive regulation of anti-apoptosis
- GO:0046318 negative regulation of glucosylceramide biosynthetic process
- GO:0046320 regulation of fatty acid oxidation
- Regulatory
- For research purposes only
Applications of AMPK Alpha 1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence | 1/50 | 1/200 | |
| Immunohistology - Paraffin | 1/50 | 1/200 | |
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti AMPK alpha 1 antibody detects a band of approximately 63 kDa in cell lysates and tissue lysates under reducing conditions
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
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