AMPK Alpha 1 antibody

Rabbit anti AMPK Alpha 1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
AMPK Alpha 1

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AHP2429
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Rabbit anti AMPK alpha 1 antibody recognizes 5’-AMP-activated protein kinase catalytic subunit alpha-1 (AMPK alpha 1), also known as AMPK1, AMPK subunit alpha-1 or PRKAA1. AMPK alpha 1 is a member of the serine/threonine protein kinase family. AMPK alpha 1 is the catalytic subunit of 5'-prime-AMP-activated protein kinase (AMPK), and is involved in the regulation of cellular energy homeostasis.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to AMPK alpha 1 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Recombinant human AMPK alpha 1
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1/50 1/200
Immunohistology - Paraffin 1/50 1/200
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti AMPK alpha 1 antibody detects a band of approximately 63 kDa in cell lysates and tissue lysates under reducing conditions

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UniProt
Q13131
Entrez Gene
PRKAA1
GO Terms
GO:0000187 activation of MAPK activity
GO:0005515 protein binding
GO:0001666 response to hypoxia
GO:0005829 cytosol
GO:0004691 cAMP-dependent protein kinase activity
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0006633 fatty acid biosynthetic process
GO:0006695 cholesterol biosynthetic process
GO:0007050 cell cycle arrest
GO:0008286 insulin receptor signaling pathway
GO:0045542 positive regulation of cholesterol biosynthetic process
GO:0045768 positive regulation of anti-apoptosis
GO:0046318 negative regulation of glucosylceramide biosynthetic process
GO:0046320 regulation of fatty acid oxidation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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