Rabbit anti AMPK alpha 1 antibody recognizes 5’-AMP-activated protein kinase catalytic subunit alpha-1 (AMPK alpha 1), also known as AMPK1, AMPK subunit alpha-1 or PRKAA1. AMPK alpha 1 is a member of the serine/threonine protein kinase family. AMPK alpha 1 is the catalytic subunit of 5'-prime-AMP-activated protein kinase (AMPK), and is involved in the regulation of cellular energy homeostasis.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to AMPK alpha 1 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Recombinant human AMPK alpha 1
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
activation of MAPK activity
response to hypoxia
cAMP-dependent protein kinase activity
metal ion binding
fatty acid biosynthetic process
cholesterol biosynthetic process
cell cycle arrest
insulin receptor signaling pathway
positive regulation of cholesterol biosynthetic process
positive regulation of anti-apoptosis
negative regulation of glucosylceramide biosynthetic process
regulation of fatty acid oxidation
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of AMPK Alpha 1 antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti AMPK alpha 1 antibody detects a band of approximately 63 kDa in cell lysates and tissue lysates under reducing conditions
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Secondary Antibodies Available
Useful Reagents Available