Amphiregulin antibody

100% Secure


Rabbit anti Human Amphiregulin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2046 IP P 50 µg
Rabbit anti Human Amphiregulin antibody recognizes human amphiregulin, also known as colorectum cell derived growth factor or CRDGF> Amphiregulin is a 252 amino acid ~28 kDa single pass transmembrane glycoprotein and ligand for the EGF receptor (Plowman et al 1990) as a mature 84 amino acid protein.

Amphiregulin has also been identified as homologous to shwannoma - derived growth factor, identified from a shwannoma derived cell line JS1 (Kimura et al. 1990).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human Amphiregulin was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
Immunogen
Synthetic peptide sequence corresponding to amino acids 8-26 of Human amphiregulin.
Approx. Protein Concentrations
Total protein concentration 0.2mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P15514 Related reagents
Entrez Gene
AREGB Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0007267 cell-cell signaling
GO:0008083 growth factor activity
GO:0008283 cell proliferation
GO:0009986 cell surface
GO:0045740 positive regulation of DNA replication
Regulatory
For research purposes only

Applications of Amphiregulin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 10ug/ml
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Human adrenal gland

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*
Histar Detection System STAR3000A 50 Tests C P
Histar Detection System STAR3000B 150 Tests C P
Histar Detection System STAR3000C 500 Tests C P