Alpha Fetoprotein antibody

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Rabbit anti Human Alpha Fetoprotein

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP457 C P 1 ml
Rabbit anti Human alpha Fetoprotein antibody recognizes human alpha fetoprotein, and shows positive staining of parenchymal cells of fetal liver and neoplastic liver cells showing yolk sac differentiation in germ cell tumours.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Pig
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human AFP were raised by repeated immunisation of rabbits with highly purified antigen
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Human Alpha-1-Fetoprotein (cord serum).

Storage Information

Storage
Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P02771 Related reagents
Entrez Gene
AFP Related reagents
GO Terms
GO:0046872 metal ion binding
GO:0006810 transport
Regulatory
For research purposes only

Applications of Alpha Fetoprotein antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/10 1/40
Immunohistology - Paraffin 1/10 1/40
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Hepalocellular carcinoma, fetal liver, germ cell tumors

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Product Specific References

References for Alpha Fetoprotein antibody

  1. Pardee, A.D. et al. (2015) Route of antigen delivery impacts the immunostimulatory activity of dendritic cell-based vaccines for hepatocellular carcinoma.
    J Immunother Cancer. 3: 32.