Alpha Elastin antibody

100% Secure

Rabbit anti Human Alpha Elastin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
4060-1060 E IF P* 1 ml
Rabbit anti Human Alpha Elastin antibody recognizes alpha and beta human elastin, also known as Tropoelastin. Elastin is a major structural protein expressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus. It is also expressed in the large arteries, lung and skin. This antibody is also known to cross react with bovine alpha elastin.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Tree shrew
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Human alpha elastin.
Approx. Protein Concentrations
IgG concentration 5.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P15502 Related reagents
Entrez Gene
ELN Related reagents
Regulatory
For research purposes only

Applications of Alpha Elastin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Immunohistology - Paraffin 1
  1. 1Fixation with 10% formalin is recommended. Brief digestion with hyaluronidase or trypsin aids antigen retrieval.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
ELISA
In a simple ELISA, with 1 µg of native antigen coated per well, the 50% OD was observed at an antibody dilution of 1/8K. Under the same conditions, the end point was observed at 1/200K.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P

Application Based External Images

Immunofluorescence

Product Specific References

References for Alpha Elastin antibody

  1. Yu, J. et al. (2010) The elastic network of articular cartilage: an immunohistochemical study of elastin fibres and microfibrils.
    J Anat. 216: 533-41.
  2. Grant, T.M. et al. (2013) Elastic fibres are broadly distributed in tendon and highly localized around tenocytes.
    J Anat. 222: 573-9.
  3. Albon, J. et al. (2007) Connective tissue structure of the tree shrew optic nerve and associated ageing changes.
    Invest Ophthalmol Vis Sci. 48: 2134-44.
  4. Zorin, V. et al. (2017) Clinical-instrumental and morphological evaluation of the effect of autologous dermal fibroblasts administration.
    J Tissue Eng Regen Med. 11 (3): 778-786.
  5. Zorin, V.L. et al. (2014) Octacalcium phosphate ceramics combined with gingiva-derived stromal cells for engineered functional bone grafts.
    Biomed Mater. 9: 055005.