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Alpha Actinin 4 antibody

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Anti Alpha Actinin 4 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1
Anti Alpha Actinin 4 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with alpha actinin 4 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 33 second exposure. Arrow points to alpha actinin 4 (molecular weight 100 kDa).

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Rabbit anti Alpha Actinin 4

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
Alpha Actinin 4

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Product Code Applications Pack Size List Price Your Price Qty
VPA00686
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Rabbit anti Human alpha actinin 4 antibody recognizes alpha actinin 4, also known as actinin-4, ACTN4, FSGS or FSGS1.

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. ACTN4 gene encodes a non-muscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in ACTN4 gene have been associated with focal and segmental glomerulosclerosis (provided by RefSeq, Jul 2008).

Rabbit anti Human alpha actinin 4 antibody detects a band of 100 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Rabbit anti alpha actinin 4 detects a band of approximately 100 kDa in MCF7 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
TRIS buffered glycine.
Preservative Stabilisers
0.01% Thiomersal
<50% Glycerol
Immunogen
Recombinant protein encompassing part of the middle region of human alpha actinin 4
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml
List Price Your Price
Description Goat anti Rabbit IgG (H/L):HRP

UniProt
O43707
Entrez Gene
ACTN4
GO Terms
GO:0005509 calcium ion binding
GO:0001882 nucleoside binding
GO:0002576 platelet degranulation
GO:0030529 ribonucleoprotein complex
GO:0051015 actin filament binding
GO:0005576 extracellular region
GO:0005178 integrin binding
GO:0005730 nucleolus
GO:0015031 protein transport
GO:0042981 regulation of apoptosis
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
GO:0031143 pseudopodium
GO:0032417 positive regulation of sodium:hydrogen antiporter activity
GO:0042803 protein homodimerization activity
GO:0043234 protein complex
GO:0048471 perinuclear region of cytoplasm
GO:0051272 positive regulation of cellular component movement

VPA00686

161118

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