Alpha Actinin 4 antibody
Rabbit anti Human alpha actinin 4 antibody recognizes alpha actinin 4, also known as actinin-4, ACTN4, FSGS or FSGS1.
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. ACTN4 gene encodes a non-muscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in ACTN4 gene have been associated with focal and segmental glomerulosclerosis (provided by RefSeq, Jul 2008).
Rabbit anti Human alpha actinin 4 antibody detects a band of 100 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. ACTN4 gene encodes a non-muscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in ACTN4 gene have been associated with focal and segmental glomerulosclerosis (provided by RefSeq, Jul 2008).
Rabbit anti Human alpha actinin 4 antibody detects a band of 100 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Rabbit anti alpha actinin 4 detects a band of approximately 100 kDa in MCF7 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- TRIS buffered glycine
- Preservative Stabilisers
- 0.01% Thiomersal
<50% Glycerol - Immunogen
- Recombinant protein encompassing part of the middle region of human alpha actinin 4
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O43707
- Entrez Gene
- ACTN4
- GO Terms
- GO:0005509 calcium ion binding
- GO:0001882 nucleoside binding
- GO:0002576 platelet degranulation
- GO:0030529 ribonucleoprotein complex
- GO:0051015 actin filament binding
- GO:0005576 extracellular region
- GO:0005178 integrin binding
- GO:0005730 nucleolus
- GO:0015031 protein transport
- GO:0042981 regulation of apoptosis
- GO:0030168 platelet activation
- GO:0031093 platelet alpha granule lumen
- GO:0031143 pseudopodium
- GO:0032417 positive regulation of sodium:hydrogen antiporter activity
- GO:0042803 protein homodimerization activity
- GO:0043234 protein complex
- GO:0048471 perinuclear region of cytoplasm
- GO:0051272 positive regulation of cellular component movement
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of Alpha Actinin 4 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
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|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | WB | 2 ml |
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