Alpha Actinin 4 antibody

100% Secure


Rabbit anti Alpha Actinin 4

Alpha Actinin 4 Antibody with Control Lysate

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00686 WB 100 µl
VPA00686KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human alpha actinin 4 antibody recognizes alpha actinin 4, also known as actinin-4, ACTN4, FSGS or FSGS1.

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. ACTN4 gene encodes a non-muscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in ACTN4 gene have been associated with focal and segmental glomerulosclerosis (provided by RefSeq, Jul 2008).

Rabbit anti Human alpha actinin 4 antibody detects a band of 100 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered glycine
Buffer Solution
TRIS buffered glycine
Preservative Stabilisers
0.01% Thiomersal
<50% Glycerol
Preservative Stabilisers
0.01% Thiomersal
<50% Glycerol
Immunogen
Recombinant protein encompassing part of the middle region of human alpha actinin 4

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
O43707 Related reagents
Entrez Gene
ACTN4 Related reagents
GO Terms
GO:0005509 calcium ion binding
GO:0001882 nucleoside binding
GO:0002576 platelet degranulation
GO:0030529 ribonucleoprotein complex
GO:0051015 actin filament binding
GO:0005576 extracellular region
GO:0005178 integrin binding
GO:0005730 nucleolus
GO:0015031 protein transport
GO:0042981 regulation of apoptosis
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
GO:0031143 pseudopodium
GO:0032417 positive regulation of sodium:hydrogen antiporter activity
GO:0042803 protein homodimerization activity
GO:0043234 protein complex
GO:0048471 perinuclear region of cytoplasm
GO:0051272 positive regulation of cellular component movement
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Alpha Actinin 4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Rabbit anti alpha actinin 4 detects a band of approximately 100 kDa in MCF7 cell lysates
Western Blotting
Rabbit anti alpha actinin 4 detects a band of approximately 100 kDa in MCF7 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB