Alkaline Phosphatase antibody

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Sheep anti Human Alkaline Phosphatase (Bap):HRP

Sheep anti Human Alkaline Phosphatase (Bap)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
0300-0578P E 1 ml
0300-0578G E 1 ml
Sheep anti Human alkaline phosphatase (Bap) antibody recognizes human bone alkaline phosphatase (BAP), a membrane-bound hydrolase enzyme expressed by osteoblast cells.

Alkaline phosphatase (AP) is a ubiquitously expressed enzyme which removes phosphate groups from target molecules, including DNA, RNA and alkaloids, under alkaline conditions, and is present at higher concentrations in the placenta (placental AP), intestines (intestinal AP) and liver/bone/kidney (tissue non-specific AP). Although the exact biochemical function of BAP is uncertain, measurement of the serum levels of BAP can be used as a biochemical indicator of bone turnover. Conditions which present with a decrease in the level of BAP (hypophosphatasemia) include the inherited bone-deforming disorder hypophosphatasia and osteoporosis, whilst an increase in BAP (hyperphosphatasemia) is associated with Paget’s disease, bone fractures and osteosarcomas.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human alkaline phosphatase were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography on protein G.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01%Thiomersal
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Alkaline phosphatase from human bone
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Approx. Protein Concentrations
IgG concentration 5.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
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Entrez Gene
ALPL Related reagents
Regulatory
For research purposes only

Applications of Alkaline Phosphatase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
ELISA 1/1500 1/15000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Product Specific References

References for Alkaline Phosphatase antibody

  1. LiuMi, a.o. et al. (2017) Fluorescent microsphere immunochromatographic assays for detecting bone alkaline phosphatase based on biolayer interferometry-selected antibody
    RSC Adv. 7 (52): 32952-9.

Further Reading

  1. Farley, J. & Baylink, D.J. (1995) Skeletal alkaline phosphatase activity in serum.
    Clin Chem. 41 (11): 1551-3.