ALK1 antibody
Rabbit anti Human ALK1 antibody recognizes serine/threonine-protein kinase receptor R3, also known as ACVRLK1.
The ACVRL1 gene encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein, sometimes termed ALK1, shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in ACVRL1 are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2 (provided by RefSeq, Jul 2008).
Rabbit anti Human ALK1 antibody detects a band of 56 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The ACVRL1 gene encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein, sometimes termed ALK1, shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in ACVRL1 are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2 (provided by RefSeq, Jul 2008).
Rabbit anti Human ALK1 antibody detects a band of 56 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Rabbit anti ALK1 detects a band of approximately 56 kDa in K562 cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thimerosal
1% Bovine Serum Albumin
<50% Glycerol - Preservative Stabilisers
- 0.01% Thimerosal
1% Bovine Serum Albumin
<50% Glycerol - Immunogen
- Recombinant protein encompassing part of the C-terminal region of human ALK1
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg K562 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P37023 Related reagents
- Entrez Gene
- ACVRL1 Related reagents
- GO Terms
- GO:0001525 angiogenesis
- GO:0004702 receptor signaling protein serine/threonine kinase activity
- GO:0005887 integral to plasma membrane
- GO:0046872 metal ion binding
- GO:0005524 ATP binding
- GO:0006468 protein phosphorylation
- GO:0007179 transforming growth factor beta receptor signaling pathway
- GO:0050431 transforming growth factor beta binding
- GO:0008217 regulation of blood pressure
- GO:0008285 negative regulation of cell proliferation
- GO:0009986 cell surface
- GO:0010596 negative regulation of endothelial cell migration
- GO:0016361 activin receptor activity, type I
- GO:0030308 negative regulation of cell growth
- GO:0030513 positive regulation of BMP signaling pathway
- GO:0035313 wound healing, spreading of epidermal cells
- GO:0045941 positive regulation of transcription
- GO:0046332 SMAD binding
- GO:0048185 activin binding
- GO:0051895 negative regulation of focal adhesion assembly
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of ALK1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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