ALK1 antibody

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Rabbit anti ALK1

ALK1 Antibody with Control Lysate

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00702 WB 100 µl
VPA00702KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human ALK1 antibody recognizes serine/threonine-protein kinase receptor R3, also known as ACVRLK1.

The ACVRL1 gene encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein, sometimes termed ALK1, shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in ACVRL1 are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2 (provided by RefSeq, Jul 2008).

Rabbit anti Human ALK1 antibody detects a band of 56 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Rabbit anti ALK1 detects a band of approximately 56 kDa in K562 cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thimerosal
1% Bovine Serum Albumin
<50% Glycerol
Preservative Stabilisers
0.01% Thimerosal
1% Bovine Serum Albumin
<50% Glycerol
Immunogen
Recombinant protein encompassing part of the C-terminal region of human ALK1
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg K562 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P37023 Related reagents
Entrez Gene
ACVRL1 Related reagents
GO Terms
GO:0001525 angiogenesis
GO:0004702 receptor signaling protein serine/threonine kinase activity
GO:0005887 integral to plasma membrane
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0006468 protein phosphorylation
GO:0007179 transforming growth factor beta receptor signaling pathway
GO:0050431 transforming growth factor beta binding
GO:0008217 regulation of blood pressure
GO:0008285 negative regulation of cell proliferation
GO:0009986 cell surface
GO:0010596 negative regulation of endothelial cell migration
GO:0016361 activin receptor activity, type I
GO:0030308 negative regulation of cell growth
GO:0030513 positive regulation of BMP signaling pathway
GO:0035313 wound healing, spreading of epidermal cells
GO:0045941 positive regulation of transcription
GO:0046332 SMAD binding
GO:0048185 activin binding
GO:0051895 negative regulation of focal adhesion assembly
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of ALK1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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