ALDOA antibody

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Anti ALDOA Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Rabbit anti Human ALDOA antibody (**VPA00226**) followed by detection with Goat anti Rabbit HRP conjugated(1/10,000,STAR208P) and visualized on the ChemiDoc MP with 25 second exposure. Arrow points to ALDOA (molecular weight 39 kDa).

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Rabbit anti ALDOA

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: ALDOA

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Product Code Applications Pack Size List Price Your Price Qty

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VPA00226 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human ALDOA antibody recognizes fructose-bisphosphate aldolase A, also known as epididymis secretory sperm binding protein Li 87p, fructose-1,6-bisphosphate triosephosphate-lyase, lung cancer antigen NY-LU-1 and muscle-type aldolase.

Encoded by the ALDOA gene, fructose-bisphosphate aldolase A is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Expression of this enzyme is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing and alternative promoter usage results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 10 (provided by RefSeq, Aug 2011).

Rabbit anti Human ALDOA antibody detects a band of 39 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti ALDOA detects a band of approximately 39 kDa in HeLa cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Rabbit Ig fraction prepared by ammonium sulphate precipitation.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: KLH conjugated synthetic peptide between 66-95 amino acids from the N-terminal region of human ALDOA
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
List Price	Your Price
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P04075
Entrez Gene: ALDOA
GO Terms: GO:0002576 platelet degranulation; GO:0003779 actin binding; GO:0004332 fructose-bisphosphate aldolase activity; GO:0005829 cytosol; GO:0006094 gluconeogenesis; GO:0006096 glycolysis; GO:0006754 ATP biosynthetic process; GO:0006941 striated muscle contraction; GO:0007015 actin filament organization; View More GO Terms; GO:0008360 regulation of cell shape; GO:0042802 identical protein binding; GO:0015629 actin cytoskeleton; GO:0015631 tubulin binding; GO:0030168 platelet activation; GO:0030388 fructose 1,6-bisphosphate metabolic process; GO:0031093 platelet alpha granule lumen; GO:0031674 I band; GO:0046716 muscle cell homeostasis; GO:0070061 fructose binding; GO:0070062 extracellular vesicular exosome