ALDOA antibody

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Rabbit anti ALDOA

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00226
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human ALDOA antibody recognizes fructose-bisphosphate aldolase A, also known as epididymis secretory sperm binding protein Li 87p, fructose-1,6-bisphosphate triosephosphate-lyase, lung cancer antigen NY-LU-1 and muscle-type aldolase.

Encoded by the ALDOA gene, fructose-bisphosphate aldolase A is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Expression of this enzyme is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing and alternative promoter usage results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 10 (provided by RefSeq, Aug 2011).

Rabbit anti Human ALDOA antibody detects a band of 39 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti ALDOA detects a band of approximately 39 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit Ig fraction prepared by ammonium sulphate precipitation
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide between 66-95 amino acids from the N-terminal region of human ALDOA

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P04075
Entrez Gene
ALDOA
GO Terms
GO:0002576 platelet degranulation
GO:0003779 actin binding
GO:0004332 fructose-bisphosphate aldolase activity
GO:0005829 cytosol
GO:0006094 gluconeogenesis
GO:0006096 glycolysis
GO:0006754 ATP biosynthetic process
GO:0006941 striated muscle contraction
GO:0007015 actin filament organization
GO:0008360 regulation of cell shape
GO:0042802 identical protein binding
GO:0015629 actin cytoskeleton
GO:0015631 tubulin binding
GO:0030168 platelet activation
GO:0030388 fructose 1,6-bisphosphate metabolic process
GO:0031093 platelet alpha granule lumen
GO:0031674 I band
GO:0046716 muscle cell homeostasis
GO:0070061 fructose binding
GO:0070062 extracellular vesicular exosome
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of ALDOA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml