Aldehyde Dehydrogenase 1 antibody

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Rabbit anti Aldehyde Dehydrogenase 1

Aldehyde dehydrogenase 1 antibody recognizes aldehyde dehydrogenase 1 also known as ALDH1.

Aldehyde Dehydrogenase 1 Antibody with Control Lysate

Aldehyde dehydrogenase 1 antibody recognizes aldehyde dehydrogenase 1 also known as ALDH1.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00867 WB 100 µl
VPA00867KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human aldehyde dehydrogenase 1 antibody recognizes aldehyde dehydrogenase 1 also known as ALDH1.

The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet (provided by RefSeq, Mar 2011).

Rabbit anti Human aldehyde dehydrongenase 1 antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
2% Sucrose
Preservative Stabilisers
0.09% Sodium Azide
2% Sucrose
Immunogen
Synthetic peptide directed corresponding to a C-terminal segment of human aldehyde dehydrogenase 1

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P00352 Related reagents
Entrez Gene
ALDH1A1 Related reagents
GO Terms
GO:0001758 retinal dehydrogenase activity
GO:0004029 aldehyde dehydrogenase (NAD) activity
GO:0005829 cytosol
GO:0005099 Ras GTPase activator activity
GO:0005497 androgen binding
GO:0006069 ethanol oxidation
GO:0006081 cellular aldehyde metabolic process
GO:0006805 xenobiotic metabolic process
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Aldehyde Dehydrogenase 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti aldehyde dehydrogenase 1 antibody recognizes a band of approximately 55 kDa in A549 cell lysates
Western Blotting
Anti aldehyde dehydrogenase 1 antibody recognizes a band of approximately 55 kDa in A549 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB