AKT2 (pSer474) antibody

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Rabbit anti AKT2 (pSer474)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2548 P WB 50 µl
Rabbit anti AKT2 (pSer474) antibody recognizes AKT2 also known as RAC-beta serine/threonine-protein kinase and protein kinase B beta, when phosphorylated at serine 474. AKT2 is a serine/threonine protein kinase that plays a key role in the PI3K-Akt signaling pathway, known to regulate cell growth, proliferation and survival. AKT2 is specifically involved in the insulin receptor signaling pathway. AKT2 is phosphorylated at serine 474 by (MAPK)-activated protein kinase-2, integrin-linked kinase, p38 MAPK, PKCα, PKCβII, mTORC2, double-stranded DNA-dependent PK, autophosphorylation and ATM. Phosphorylation at threonine 309 and serine 474 are required for full activation of AKT2 (Suizu et al. 2009).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to AKT2 (pSer474) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 474 of human AKT2

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P31751 Related reagents
Entrez Gene
AKT2 Related reagents
GO Terms
GO:0005515 protein binding
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0008286 insulin receptor signaling pathway
GO:0010748 negative regulation of plasma membrane long-chain fatty acid transport
GO:0046326 positive regulation of glucose import
GO:0032000 positive regulation of fatty acid beta-oxidation
GO:0045725 positive regulation of glycogen biosynthetic process
Regulatory
For research purposes only

Applications of AKT2 (pSer474) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/50 1/100
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Suizu, F. et al. (2009) The E3 ligase TTC3 facilitates ubiquitination and degradation of phosphorylated Akt
    Dev Cell. 17 (6): 800-10.