AKT1 (pTyr315)/AKT2 (pTyr316)/AKT3 (pTyr312) antibody

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Rabbit anti AKT1 (pTyr315)/AKT2 (pTyr316)/AKT3 (pTyr312)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2547 WB 50 µl
Rabbit anti AKT1 (pTyr315)/AKT2 (pTyr316)/AKT3 (pTyr312) antibody recognizes AKT1 when phosphorylated at tyrosine 315, AKT2 when phosphorylated at tyrosine 316 and AKT3 when phosphorylated at tyrosine 312. AKT 1, 2 and 3 are serine/threonine protein kinases that play a key role in the PI3K-Akt signaling pathway, known to regulate cell growth, proliferation and survival. AKT1 is phosphorylated at tyrosine 315 (corresponding to tyrosine 316/312 of AKT2/AKT3 respectively) by Src and PTK6, tyrosine phosphorylation is important for growth factor mediated activation of AKT (Zheng et al. 2010).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to AKT1 (pTyr315)/AKT2 (pTyr316)/AKT3 (pTyr312) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding tyrosine 315 of human AKT1/ tyrosine 316 of human AKT2/ tyrosine 312 of human AKT3

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P31749 Related reagents
P31751 Related reagents
Q9Y243 Related reagents
Entrez Gene
AKT1 Related reagents
AKT2 Related reagents
AKT3 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0000060 protein import into nucleus, translocation
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0005547 phosphatidylinositol-3,4,5-trisphosphate binding
GO:0005654 nucleoplasm
GO:0005978 glycogen biosynthetic process
GO:0006417 regulation of translation
GO:0006469 negative regulation of protein kinase activity
GO:0006809 nitric oxide biosynthetic process
GO:0006924 activation-induced cell death of T cells
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0008286 insulin receptor signaling pathway
GO:0008629 induction of apoptosis by intracellular signals
GO:0008633 activation of pro-apoptotic gene products
GO:0042802 identical protein binding
GO:0009408 response to heat
GO:0010748 negative regulation of plasma membrane long-chain fatty acid transport
GO:0010975 regulation of neuron projection development
GO:0016071 mRNA metabolic process
GO:0046326 positive regulation of glucose import
GO:0046889 positive regulation of lipid biosynthetic process
GO:0019899 enzyme binding
GO:0018105 peptidyl-serine phosphorylation
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030168 platelet activation
GO:0030235 nitric-oxide synthase regulator activity
GO:0030307 positive regulation of cell growth
GO:0031018 endocrine pancreas development
GO:0031295 T cell costimulation
GO:0031659 positive regulation of cyclin-dependent protein kinase activity involved in G1/S
GO:0031999 negative regulation of fatty acid beta-oxidation
GO:0034405 response to fluid shear stress
GO:0043325 phosphatidylinositol-3,4-bisphosphate binding
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0045600 positive regulation of fat cell differentiation
GO:0045725 positive regulation of glycogen biosynthetic process
GO:0046777 protein autophosphorylation
GO:0048009 insulin-like growth factor receptor signaling pathway
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051000 positive regulation of nitric-oxide synthase activity
GO:0070141 response to UV-A
GO:0090004 positive regulation of establishment of protein localization in plasma membrane
GO:0005515 protein binding
GO:0005634 nucleus
GO:0032000 positive regulation of fatty acid beta-oxidation
GO:0007165 signal transduction
GO:0005794 Golgi apparatus
GO:0006468 protein phosphorylation
Regulatory
For research purposes only

Applications of AKT1 (pTyr315)/AKT2 (pTyr316)/AKT3 (pTyr312) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Zheng, Y. et al. (2010) Protein tyrosine kinase 6 directly phosphorylates AKT and promotes AKT activation in response to epidermal growth factor
    Mol Cell Biol. 30 (17): 4280-92.