AKT1 (pThr450) antibody

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WB ResetRabbit anti AKT1 (pThr450)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- AKT1 (pThr450)
Rabbit anti AKT1 (pThr450) antibody recognizes AKT1, also known as RAC-alpha serine/threonine-protein kinase, protein kinase B alpha or Proto-oncogene c-Akt, when phosphorylated at threonine 450. AKT1 is a 480 amino acid ~48 kDa protein kinase that play a key role in the PI3K-Akt signaling pathway, known to regulate cell growth, proliferation and survival. Phosphorylation of threonine 308 and serine 473 are required for full activation of AKT1. Newly synthesized Akt is suggested to require phosphorylation at threonine 450 for proper protein folding (Liao and Hung 2010). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to AKT1 (pThr450) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.05% Proclin™300
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding threonine 450 of human AKT1
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- ProClin™ is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Western Blotting | ![]() |
1/500 | 1/1000 |
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- UniProt
- P31749
- Entrez Gene
- AKT1
- GO Terms
- GO:0005886 plasma membrane
- GO:0000060 protein import into nucleus, translocation
- GO:0005829 cytosol
- GO:0004674 protein serine/threonine kinase activity
- GO:0005524 ATP binding
- GO:0005547 phosphatidylinositol-3,4,5-trisphosphate binding
- GO:0005654 nucleoplasm
- GO:0005978 glycogen biosynthetic process
- GO:0006417 regulation of translation
- View More GO Terms
- GO:0006469 negative regulation of protein kinase activity
- GO:0006809 nitric oxide biosynthetic process
- GO:0006924 activation-induced cell death of T cells
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0008286 insulin receptor signaling pathway
- GO:0008629 induction of apoptosis by intracellular signals
- GO:0008633 activation of pro-apoptotic gene products
- GO:0042802 identical protein binding
- GO:0009408 response to heat
- GO:0010748 negative regulation of plasma membrane long-chain fatty acid transport
- GO:0010975 regulation of neuron projection development
- GO:0016071 mRNA metabolic process
- GO:0046326 positive regulation of glucose import
- GO:0046889 positive regulation of lipid biosynthetic process
- GO:0019899 enzyme binding
- GO:0018105 peptidyl-serine phosphorylation
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0030168 platelet activation
- GO:0030235 nitric-oxide synthase regulator activity
- GO:0030307 positive regulation of cell growth
- GO:0031018 endocrine pancreas development
- GO:0031295 T cell costimulation
- GO:0031659 positive regulation of cyclin-dependent protein kinase activity involved in G1/S
- GO:0031999 negative regulation of fatty acid beta-oxidation
- GO:0034405 response to fluid shear stress
- GO:0043325 phosphatidylinositol-3,4-bisphosphate binding
- GO:0045429 positive regulation of nitric oxide biosynthetic process
- GO:0045600 positive regulation of fat cell differentiation
- GO:0045725 positive regulation of glycogen biosynthetic process
- GO:0046777 protein autophosphorylation
- GO:0048009 insulin-like growth factor receptor signaling pathway
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0051000 positive regulation of nitric-oxide synthase activity
- GO:0070141 response to UV-A
- GO:0090004 positive regulation of establishment of protein localization in plasma membrane
AHP2546


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