AIF antibody

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Rabbit anti AIF

AIF antibody recognizes apoptosis-inducing factor 1, mitochondrial, also known as programmed cell death protein 8. The antibody has been extensively validated for western blotting using whole cell lysates.

AIF Antibody with Control Lysate

AIF antibody recognizes apoptosis-inducing factor 1, mitochondrial, also known as programmed cell death protein 8. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00017 WB 100 µl
VPA00017KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human AIF antibody recognizes human apoptosis-inducing factor (AIF), also known as human apoptosis-inducing factor 1, mitochondrial and programmed cell death protein 8. AIF is a 613 amino acid member of the FAD-dependent oxidoreductase family. Rabbit anti Human AIF antibody reacts with an internal region of AIF (within the region aa 517-531). AIF is widely expressed in human tissues and localizes in the mitochondria. AIF translocates to the nucleus upon induction of apoptosis. Multiple isoforms of AIF are generated by alternative splicing. Rabbit anti Human AIF antibody recognizes isoforms 1, 2, 3 and 5. It is not expected to recognize isoform 4 or 6 as they lack the immunogen sequence.

Mutations in the AIFM1 gene can result in various disease conditions including COXPD6 and Cowchock syndrome.

Rabbit anti Human AIF antibody recognizes human AIF as a single band of ~67 kDa in multiple human cell line lysates and cross reacts with rodent cell line lysates tested.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by ion exchange chromatography
Preparation
20μl Rabbit polyclonal antibody purified by ion exchange chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Peptide corresponding to amino acids 517 - 531 of human AIF

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
O95831 Related reagents
Entrez Gene
AIFM1 Related reagents
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of AIF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti AIF antibody detects a band of approximately 67 kDa in HEK293 cell lysates.
Western Blotting
Anti AIF antibody detects a band of approximately 67 kDa in HEK293 cell lysates.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB