AIF antibody
Rabbit anti AIF
AIF antibody recognizes apoptosis-inducing factor 1, mitochondrial, also known as programmed cell death protein 8. The antibody has been extensively validated for western blotting using whole cell lysates.
AIF Antibody with Control Lysate
AIF antibody recognizes apoptosis-inducing factor 1, mitochondrial, also known as programmed cell death protein 8. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00017 | WB | 100 µl | ||||
| VPA00017KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human AIF antibody recognizes human apoptosis-inducing factor (AIF), also known as human apoptosis-inducing factor 1, mitochondrial and programmed cell death protein 8. AIF is a 613 amino acid member of the FAD-dependent oxidoreductase family. Rabbit anti Human AIF antibody reacts with an internal region of AIF (within the region aa 517-531). AIF is widely expressed in human tissues and localizes in the mitochondria. AIF translocates to the nucleus upon induction of apoptosis. Multiple isoforms of AIF are generated by alternative splicing. Rabbit anti Human AIF antibody recognizes isoforms 1, 2, 3 and 5. It is not expected to recognize isoform 4 or 6 as they lack the immunogen sequence.
Mutations in the AIFM1 gene can result in various disease conditions including COXPD6 and Cowchock syndrome.
Rabbit anti Human AIF antibody recognizes human AIF as a single band of ~67 kDa in multiple human cell line lysates and cross reacts with rodent cell line lysates tested.
Mutations in the AIFM1 gene can result in various disease conditions including COXPD6 and Cowchock syndrome.
Rabbit anti Human AIF antibody recognizes human AIF as a single band of ~67 kDa in multiple human cell line lysates and cross reacts with rodent cell line lysates tested.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by ion exchange chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by ion exchange chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Peptide corresponding to amino acids 517 - 531 of human AIF
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- O95831 Related reagents
- Entrez Gene
- AIFM1 Related reagents
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of AIF antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
- Western Blotting
- Anti AIF antibody detects a band of approximately 67 kDa in HEK293 cell lysates.
- Western Blotting
- Anti AIF antibody detects a band of approximately 67 kDa in HEK293 cell lysates.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
