AIF antibody

Rabbit anti AIF

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
AIF

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VPA00017
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Rabbit anti Human AIF antibody recognizes human apoptosis-inducing factor (AIF), also known as human apoptosis-inducing factor 1, mitochondrial and programmed cell death protein 8. AIF is a 613 amino acid member of the FAD-dependent oxidoreductase family. Rabbit anti Human AIF antibody reacts with an internal region of AIF (within the region aa 517-531). AIF is widely expressed in human tissues and localizes in the mitochondria. AIF translocates to the nucleus upon induction of apoptosis. Multiple isoforms of AIF are generated by alternative splicing. Rabbit anti Human AIF antibody recognizes isoforms 1, 2, 3 and 5. It is not expected to recognize isoform 4 or 6 as they lack the immunogen sequence.

Mutations in the AIFM1 gene can result in various disease conditions including COXPD6 and Cowchock syndrome.

Rabbit anti Human AIF antibody recognizes human AIF as a single band of ~67 kDa in multiple human cell line lysates and cross reacts with rodent cell line lysates tested.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti AIF antibody detects a band of approximately 67 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by ion exchange chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Peptide corresponding to amino acids 517 - 531 of human AIF
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
O95831
Entrez Gene
AIFM1
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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