AIF antibody

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Rabbit anti Human AIF (aa109-122)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP579 WB* 0.1 mg
Rabbit anti Human AIF antibody recognizes human apoptosis-inducing factor 1, mitochondrial, also known as AIF or programmed cell death protein 8. Human AIF is a 613 amino acid mitochondrial protein bearing a 54 amino acid N-terminal transit peptide and a 47 amino acid propeptide region adjacent. The mature peptide is a 512 amino acid ~67 kDa mitochondrial intermembrane space protein formed during apoptosis. Rabbit anti Human AIF antibody reacts with the N-terminal region of the mature protein. AIF is widely expressed in human tissues and is released to the cytoplasm and then translocates to the nucleus on certain death signals where it is involved in the induction of nuclear apoptosis (Kim et al. 2006).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to AIF (NT) were raised by repeated immunisation of rabbit with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
Peptide corresponding to amino acids 109 - 122 of human AIF (NT).
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
O95831 Related reagents
Entrez Gene
AIFM1 Related reagents
Regulatory
For research purposes only

Applications of AIF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1 1 / 500
  1. 1AHP579 is suitable for western blotting under non-reducing conditions only.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Product Specific References

Further Reading

  1. Susin. S.A. et al. (1999) Molecular characterization of mitochondrial apoptosis - inducing factor.
    Nature. 397: 441-6.