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AID antibody

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Anti AID (C-Terminal) Antibody thumbnail image 1 Anti AID (C-Terminal) Antibody thumbnail image 2
Anti AID (C-Terminal) Antibody gallery image 1
Western blot analysis of whole cell lysate from Ramos human Burkitt's lymphoma cell line probed with Rabbit anti Human AID (AHP994) in the absence (A) or presence (B) of blocking peptide.
Anti AID (C-Terminal) Antibody gallery image 2
Immunocytochemical staining of Ramos cells with Rabbit anti Human AID (AHP994)

Rabbit anti AID (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP994T
Datasheet
WB
SDS
50 µg loader

Rabbit anti Human AID antibody recognizes an epitope within the C-terminal region (CT) of Activation-induced cytidine deaminase (AID), a ~24 kDa homologue of the RNA-editing cytidine deaminase enzyme, APOBEC-1.

Although the molecular mechanisms by which AID acts remain unclear, a growing number of studies reveal a requirement for AID during somatic hypermutation (SHM) and class switch recombination (CSR). These genetic modification events take place within highly specialised B cell germinal centres, during the final maturation stages of B cells, giving rise to high-affinity antibodies which use CSR to switch from IgM to IgG, IgA or IgE isotypes.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Antisera to human AID (CT) were raised by repeated immunisation of rabbits with highly purified antigen.
Purified IgG pepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A 14 amino acid peptide located near human AID carboxy-terminus.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q9GZX7
Entrez Gene
AICDA
GO Terms
GO:0005515 protein binding
GO:0004126 cytidine deaminase activity
GO:0005737 cytoplasm
GO:0006397 mRNA processing
GO:0008270 zinc ion binding
GO:0030183 B cell differentiation
Regulatory
For research purposes only

Applications of AID antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP994T detects a band of approximately 24kDa in Ramos whole cell lysates.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Product Specific References

Further Reading

  1. Muramatsu, M. et al. (1999) Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells.
    J Biol Chem. 274 (26): 18470-6.
  2. Muramatsu, M. et al. (2000) Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme.
    Cell. 102 (5): 553-63.
  3. Yu, K. et al. (2005) Fine-structure analysis of activation-induced deaminase accessibility to class switch region R-loops.
    Mol Cell Biol. 25 (5): 1730-6.

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