AID antibody
Rabbit anti Human AID antibody recognizes an epitope within the C-terminal region (CT) of Activation-induced cytidine deaminase (AID), a ~24 kDa homologue of the RNA-editing cytidine deaminase enzyme, APOBEC-1.
Although the molecular mechanisms by which AID acts remain unclear, a growing number of studies reveal a requirement for AID during somatic hypermutation (SHM) and class switch recombination (CSR). These genetic modification events take place within highly specialised B cell germinal centres, during the final maturation stages of B cells, giving rise to high-affinity antibodies which use CSR to switch from IgM to IgG, IgA or IgE isotypes.
Although the molecular mechanisms by which AID acts remain unclear, a growing number of studies reveal a requirement for AID during somatic hypermutation (SHM) and class switch recombination (CSR). These genetic modification events take place within highly specialised B cell germinal centres, during the final maturation stages of B cells, giving rise to high-affinity antibodies which use CSR to switch from IgM to IgG, IgA or IgE isotypes.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Antisera to human AID (CT) were raised by repeated immunisation of rabbits with highly purified antigen.
Purified IgG pepared by affinity chromatography. - Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Immunogen
- A 14 amino acid peptide located near human AID carboxy-terminus.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9GZX7
- Entrez Gene
- AICDA
- GO Terms
- GO:0005515 protein binding
- GO:0004126 cytidine deaminase activity
- GO:0005737 cytoplasm
- GO:0006397 mRNA processing
- GO:0008270 zinc ion binding
- GO:0030183 B cell differentiation
- Regulatory
- For research purposes only
Applications of AID antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP994T detects a band of approximately 24kDa in Ramos whole cell lysates.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
Product Specific References
Further Reading
-
Muramatsu, M. et al. (1999) Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells.
J Biol Chem. 274 (26): 18470-6. -
Muramatsu, M. et al. (2000) Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme.
Cell. 102 (5): 553-63. -
Yu, K. et al. (2005) Fine-structure analysis of activation-induced deaminase accessibility to class switch region R-loops.
Mol Cell Biol. 25 (5): 1730-6.
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