Rabbit anti Human AID antibody recognizes an epitope within the C-terminal region (CT) of Activation-induced cytidine deaminase (AID), a ~24 kDa homologue of the RNA-editing cytidine deaminase enzyme, APOBEC-1.
Although the molecular mechanisms by which AID acts remain unclear, a growing number of studies reveal a requirement for AID during somatic hypermutation (SHM) and class switch recombination (CSR). These genetic modification events take place within highly specialised B cell germinal centres, during the final maturation stages of B cells, giving rise to high-affinity antibodies which use CSR to switch from IgM to IgG, IgA or IgE isotypes.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antisera to human AID (CT) were raised by repeated immunisation of rabbits with highly purified antigen.
Purified IgG pepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- A 14 amino acid peptide located near human AID carboxy-terminus.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
cytidine deaminase activity
zinc ion binding
B cell differentiation
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of AID antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP994T detects a band of approximately 24kDa in Ramos whole cell lysates.
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Secondary Antibodies Available
Product Specific References
Muramatsu, M. et al. (1999) Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells.
J Biol Chem. 274 (26): 18470-6.
Muramatsu, M. et al. (2000) Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme.
Cell. 102 (5): 553-63.
Yu, K. et al. (2005) Fine-structure analysis of activation-induced deaminase accessibility to class switch region R-loops.
Mol Cell Biol. 25 (5): 1730-6.
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