AHR antibody
Rabbit anti Human AHR antibody recognises human aryl hydrocarbon receptor (AHR), a ~96 kDa ubiquitously expressed ligand-activated transcription factor. AHR binds planar aromatic hydrocarbons and plays a role in regulating biological responses to them. It activates expression of xenobiotic chemical metabolising enzyme genes such as cytochrome P450. AHR is also involved in cell-cycle regulation and is thought to be involved in tissue maturation.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Antiserum Preparation
- Antisera to human AHR were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Synthetic human AHR.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P35869 Related reagents
- Entrez Gene
- AHR Related reagents
- GO Terms
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0006915 apoptosis
- GO:0004879 ligand-dependent nuclear receptor activity
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0006366 transcription from RNA polymerase II promoter
- GO:0006950 response to stress
- GO:0007049 cell cycle
- GO:0008134 transcription factor binding
- GO:0009410 response to xenobiotic stimulus
- GO:0051879 Hsp90 protein binding
- GO:0010843 promoter binding
- GO:0030528 transcription regulator activity
- GO:0030888 regulation of B cell proliferation
- Regulatory
- For research purposes only
Applications of AHR antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Respitory epithelium and bronchial smooth muscle.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | 500 ml | P | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025C | 50 ml | P |
Product Specific References
References for AHR antibody
-
Tomokiyo, A. et al. (2012) Alternation of extracellular matrix remodeling and apoptosis by activation of the aryl hydrocarbon receptor pathway in human periodontal ligament cells.
J Cell Biochem. 113 (10): 3093-103. -
Dobrzyński, M. et al. (2017) Tooth development disorders in infants of rat dams exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin and protective role of tocopherol and acetylsalicylic acid.
Pol J Vet Sci. 20 (4): 769-78.
Further Reading
-
Puga, A. et al. (2002) Role of the aryl hydrocarbon receptor in cell cycle regulation.
Chem Biol Interact. 141 (1-2): 117-30. -
Dale, Y.R. & Eltom, S.E. (2006) Calpain mediates the dioxin-induced activation and down-regulation of the aryl hydrocarbon receptor.
Mol Pharmacol. 70 (5): 1481-7.
