Rabbit anti Human AHR antibody recognises human aryl hydrocarbon receptor (AHR), a ~96 kDa ubiquitously expressed ligand-activated transcription factor. AHR binds planar aromatic hydrocarbons and plays a role in regulating biological responses to them. It activates expression of xenobiotic chemical metabolising enzyme genes such as cytochrome P450. AHR is also involved in cell-cycle regulation and is thought to be involved in tissue maturation.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography
- Antiserum Preparation
- Antisera to human AHR were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Synthetic human AHR.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
sequence-specific DNA binding transcription factor activity
ligand-dependent nuclear receptor activity
transcription from RNA polymerase II promoter
response to stress
transcription factor binding
response to xenobiotic stimulus
Hsp90 protein binding
transcription regulator activity
regulation of B cell proliferation
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of AHR antibody
|Immunohistology - Paraffin 1
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Respitory epithelium and bronchial smooth muscle.
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
References for AHR antibody
Tomokiyo, A. et al. (2012) Alternation of extracellular matrix remodeling and apoptosis by activation of the aryl hydrocarbon receptor pathway in human periodontal ligament cells.
J Cell Biochem. 113 (10): 3093-103.
Dobrzyński, M. et al. (2017) Tooth development disorders in infants of rat dams exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin and protective role of tocopherol and acetylsalicylic acid.
Pol J Vet Sci. 20 (4): 769-78.
Puga, A. et al. (2002) Role of the aryl hydrocarbon receptor in cell cycle regulation.
Chem Biol Interact. 141 (1-2): 117-30.
Dale, Y.R. & Eltom, S.E. (2006) Calpain mediates the dioxin-induced activation and down-regulation of the aryl hydrocarbon receptor.
Mol Pharmacol. 70 (5): 1481-7.
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