AHR antibody

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Rabbit anti Human AHR

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1107 P* 50 µg
Rabbit anti Human AHR antibody recognises human aryl hydrocarbon receptor (AHR), a ~96 kDa ubiquitously expressed ligand-activated transcription factor. AHR binds planar aromatic hydrocarbons and plays a role in regulating biological responses to them. It activates expression of xenobiotic chemical metabolising enzyme genes such as cytochrome P450. AHR is also involved in cell-cycle regulation and is thought to be involved in tissue maturation.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Antiserum Preparation
Antisera to human AHR were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Synthetic human AHR.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P35869 Related reagents
Entrez Gene
AHR Related reagents
GO Terms
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0006915 apoptosis
GO:0004879 ligand-dependent nuclear receptor activity
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0006366 transcription from RNA polymerase II promoter
GO:0006950 response to stress
GO:0007049 cell cycle
GO:0008134 transcription factor binding
GO:0009410 response to xenobiotic stimulus
GO:0051879 Hsp90 protein binding
GO:0010843 promoter binding
GO:0030528 transcription regulator activity
GO:0030888 regulation of B cell proliferation
Regulatory
For research purposes only

Applications of AHR antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Respitory epithelium and bronchial smooth muscle.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P

Product Specific References

References for AHR antibody

  1. Tomokiyo, A. et al. (2012) Alternation of extracellular matrix remodeling and apoptosis by activation of the aryl hydrocarbon receptor pathway in human periodontal ligament cells.
    J Cell Biochem. 113 (10): 3093-103.
  2. Dobrzyński, M. et al. (2017) Tooth development disorders in infants of rat dams exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin and protective role of tocopherol and acetylsalicylic acid.
    Pol J Vet Sci. 20 (4): 769-78.

Further Reading

  1. Puga, A. et al. (2002) Role of the aryl hydrocarbon receptor in cell cycle regulation.
    Chem Biol Interact. 141 (1-2): 117-30.
  2. Dale, Y.R. & Eltom, S.E. (2006) Calpain mediates the dioxin-induced activation and down-regulation of the aryl hydrocarbon receptor.
    Mol Pharmacol. 70 (5): 1481-7.