AES antibody

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Rabbit anti AES (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1006 C WB 0.1 mg
Rabbit anti Human AES1 (C-terminal) antibody recognizes an epitope within the C-Terminal region (CT) of the 197-amino acid protein AES (amino-Terminal enhancer of split), a member of the Groucho/transducin-like enhancer of split (TLE) family of transcriptional regulators, homologous to the amino-Terminal domain of Drosophila Groucho protein.

The detachment of integrin from the extracellular matrix (ECM), results in loss of cell adhesion and cell death, termed anoikis. This type of apoptosis is controlled by integrin-mediated signaling pathways and that the mitochondrial protein Bit1 (Bcl-2 inhibitor of transcription), released into the cytoplasm during apoptosis, forms a complex with AES, to induce cell death. The effects of the interaction between Bit1 and AES can be counteracted by the attachment of cells to fibronectin through integrin α5β1.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Human AES (CT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide (NaN3)
Immunogen
A 16 amino acid peptide located near human AES carboxy-terminus.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q08117 Related reagents
Entrez Gene
AES Related reagents
GO Terms
GO:0005515 protein binding
GO:0003714 transcription corepressor activity
GO:0016055 Wnt receptor signaling pathway
GO:0005634 nucleus
GO:0009887 organ morphogenesis
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0032091 negative regulation of protein binding
GO:0060761 negative regulation of response to cytokine stimulus
GO:0070555 response to interleukin-1
GO:0090090 negative regulation of canonical Wnt receptor signaling pathway
Regulatory
For research purposes only

Applications of AES antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Western Blotting 1ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1006 detects a band of approximately 22kDa in human 293 cell lysates

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Miyasaka, H. et al. (1993) Molecular cloning and expression of mouse and human cDNA encoding AES and ESG proteins with strong similarity to Drosophila enhancer of split groucho protein.
    Eur. J. Biochem. 216: 343-352.
  2. Jan, Y. et al. (2004) A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and Groucho/TLE corepressors.
    Cell. 116: 751-762.
  3. Tetsuka, T. et al. (2000) Inhibition of nuclear factor-kappaB-mediated transcription by association with the amino-terminal enhancer of split, a Groucho-related protein lacking WD40 repeats*.
    J. Biol. Chem. 275: 4383-4390.