ADRM1 antibody

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Rabbit anti ADRM1

Rabbit anti-human ADRM1 antibody recognizes proteasomal ubiquitin receptor ADRM1, also known as 110 kDa cell membrane glycoprotein, M(r) 110,000 surface antigen, proteasome regulatory particle non-ATPase 13 or Rpn-13 homolog.

ADRM1 Antibody with Control Lysate

Product Type: PrecisionAb™ Polyclonal
Isotype: Polyclonal IgG
Format: Purified

Product Code	Applications	Pack Size
VPA00594	WB	100 µl
VPA00594KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Rabbit anti Human ADRM1 antibody recognizes the proteasomal ubiquitin receptor ADRM1, also known as 110 kDa cell membrane glycoprotein, proteasome regulatory particle non-ATPase 13, rpn13 homolog or Adhesion-regulating molecule 1.

ADRM1 gene encodes a member of the adhesion regulating molecule 1 protein family. The encoded protein is a component of the proteasome where it acts as a ubiquitin receptor and recruits the deubiquitinating enzyme, ubiquitin carboxyl-terminal hydrolase L5. Increased levels of the encoded protein are associated with increased cell adhesion, which is likely an indirect effect of this intracellular protein. Dysregulation of ADRM1 has been implicated in carcinogenesis. Alternative splicing results in multiple transcript variants (provided by RefSeq, Jul 2013).

Rabbit anti Human ADRM1 antibody detects a band of 45 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti ADRM1 detects a band of approximately 45 kDa in HeLa cell lysates.
Product Form: Purified IgG - liquid.
Product Form: Purified IgG - liquid.
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Preparation: 20μl Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
2% Sucrose.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
2% Sucrose.
Immunogen: Synthetic peptide directed towards the C terminal region of human ADRM1
Instructions For Use: Please refer to the PrecisionAb western blotting protocol.

Positive Control Lysate

Lysate Composition: 400μg HeLa lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: Q16186 Related reagents
Entrez Gene: ADRM1 Related reagents
GO Terms: GO:0000502 proteasome complex; GO:0002020 protease binding; GO:0005887 integral to plasma membrane; GO:0005624 membrane fraction; GO:0005634 nucleus; GO:0005737 cytoplasm; GO:0006368 transcription elongation from RNA polymerase II promoter; GO:0061133 endopeptidase activator activity; GO:0043248 proteasome assembly; GO:0070628 proteasome binding
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only.

Applications of ADRM1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Rabbit IgG (H/L):HRP	STAR208P	2 ml	WB
Goat anti Rabbit IgG (H/L):HRP	STAR208P	2 ml	WB

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