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ADHFE1 antibody

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Western Blot Validated
Anti ADHFE1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with ADHFE1 antibody followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 62 second exposure. Arrow points to ADHFE1 (molecular weight 50 kDa)

Rabbit anti ADHFE1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VPA00299
Datasheet
WB
SDS
100 µl loader

Rabbit anti Human ADHFE1 antibody recognizes ADHFE1 also known as iron-containing alcohol dehydrogenase 1, alcohol dehydrogenase 8 and hydroxyacid-oxoacid transhydrogenase, mitochondrial.

The ADHFE1 gene encodes hydroxyacid-oxoacid transhydrogenase (EC 1.1.99.24), which is responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues (Kardon et al. 2006).

Rabbit anti Human ADHFE1 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using human tissue and whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti ADHFE1 detects a band of approximately 50 kDa in human liver tissue
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide corresponding to the central region of human ADHFE1

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q8IWW8
Entrez Gene
ADHFE1
GO Terms
GO:0046872 metal ion binding
GO:0005759 mitochondrial matrix
GO:0006103 2-oxoglutarate metabolic process
GO:0055114 oxidation-reduction process
GO:0015993 molecular hydrogen transport
GO:0047988 hydroxyacid-oxoacid transhydrogenase activity
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of ADHFE1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml

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A Precise Solution to Reproducible Western Blots - PrecisionAb Antibodies
Request a different product with the ADHFE1 specificity