ADHFE1 antibody

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Rabbit anti ADHFE1

Rabbit anti human ADHFE1 antibody recognizes hydroxyacid-oxoacid transhydrogenase, mitochondrial, also known as Fe-containing alcohol dehydrogenase 1, alcohol dehydrogenase 8 or alcohol dehydrogenase iron-containing protein 1. This antibody has been extensively validated for western blotting.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00299 WB 100 µl
Rabbit anti Human ADHFE1 antibody recognizes ADHFE1 also known as iron-containing alcohol dehydrogenase 1, alcohol dehydrogenase 8 and hydroxyacid-oxoacid transhydrogenase, mitochondrial.

The ADHFE1 gene encodes hydroxyacid-oxoacid transhydrogenase (EC 1.1.99.24), which is responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues (Kardon et al. 2006).

Rabbit anti Human ADHFE1 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using human tissue and whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti ADHFE1 detects a band of approximately 50 kDa in human liver tissue
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide corresponding to the central region of human ADHFE1
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q8IWW8 Related reagents
Entrez Gene
ADHFE1 Related reagents
GO Terms
GO:0046872 metal ion binding
GO:0005759 mitochondrial matrix
GO:0006103 2-oxoglutarate metabolic process
GO:0055114 oxidation-reduction process
GO:0015993 molecular hydrogen transport
GO:0047988 hydroxyacid-oxoacid transhydrogenase activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of ADHFE1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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