ADHFE1 antibody
Rabbit anti ADHFE1
Rabbit anti human ADHFE1 antibody recognizes hydroxyacid-oxoacid transhydrogenase, mitochondrial, also known as Fe-containing alcohol dehydrogenase 1, alcohol dehydrogenase 8 or alcohol dehydrogenase iron-containing protein 1. This antibody has been extensively validated for western blotting.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00299 | WB | 100 µl |
Rabbit anti Human ADHFE1 antibody recognizes ADHFE1 also known as iron-containing alcohol dehydrogenase 1, alcohol dehydrogenase 8 and hydroxyacid-oxoacid transhydrogenase, mitochondrial.
The ADHFE1 gene encodes hydroxyacid-oxoacid transhydrogenase (EC 1.1.99.24), which is responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues (Kardon et al. 2006).
Rabbit anti Human ADHFE1 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using human tissue and whole cell lysates.
The ADHFE1 gene encodes hydroxyacid-oxoacid transhydrogenase (EC 1.1.99.24), which is responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues (Kardon et al. 2006).
Rabbit anti Human ADHFE1 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using human tissue and whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti ADHFE1 detects a band of approximately 50 kDa in human liver tissue
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Immunogen
- Synthetic peptide corresponding to the central region of human ADHFE1
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q8IWW8 Related reagents
- Entrez Gene
- ADHFE1 Related reagents
- GO Terms
- GO:0046872 metal ion binding
- GO:0005759 mitochondrial matrix
- GO:0006103 2-oxoglutarate metabolic process
- GO:0055114 oxidation-reduction process
- GO:0015993 molecular hydrogen transport
- GO:0047988 hydroxyacid-oxoacid transhydrogenase activity
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of ADHFE1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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