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ACTN3 antibody

Rabbit anti ACTN3

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
ACTN3

Product Code Applications Pack Size List Price Your Price Qty
VPA00529
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SDS Safety Datasheet SDS
WB 100 µl loader
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Rabbit anti Human ACTN3 antibody recognizes ACTN3 also known as alpha-actinin-3, F-actin cross-linking protein, alpha-actinin skeletal muscle isoform 3.

ACTN3 gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in ACTN3 results in both coding and non-coding variants. The reference genome contains the non-coding allele. The non-functional allele of ACTN3 is associated with elite athlete status (provided by RefSeq, Apr 2012).

Rabbit anti Human ACTN3 antibody detects a band of 103 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti ACTN3 detects a band of approximately 103 kDa in NIH/3T3 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose.
Immunogen
Synthetic peptide directed towards the N terminal region of human ACTN3
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
Q08043
Entrez Gene
ACTN3
GO Terms
GO:0005509 calcium ion binding
GO:0003779 actin binding
GO:0005829 cytosol
GO:0005178 integrin binding
GO:0005884 actin filament
GO:0005925 focal adhesion
GO:0008307 structural constituent of muscle
GO:0042981 regulation of apoptosis
GO:0030049 muscle filament sliding
GO:0031143 pseudopodium
GO:0042803 protein homodimerization activity
GO:0048041 focal adhesion assembly

VPA00529

160712

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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