ACTN3 antibody
Rabbit anti ACTN3
Rabbit anti-human ACTN3 antibody recognizes alpha-actinin-3, also known as F-actin cross-linking protein or alpha-actinin skeletal muscle. This antibody has been extensively validated for western blotting using whole cell lysates.
ACTN3 Antibody with Control Lysate
Rabbit anti-human ACTN3 antibody recognizes alpha-actinin-3, also known as F-actin cross-linking protein or alpha-actinin skeletal muscle. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00529 | WB | 100 µl | ||||
| VPA00529KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human ACTN3 antibody recognizes ACTN3 also known as alpha-actinin-3, F-actin cross-linking protein, alpha-actinin skeletal muscle isoform 3.
ACTN3 gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in ACTN3 results in both coding and non-coding variants. The reference genome contains the non-coding allele. The non-functional allele of ACTN3 is associated with elite athlete status (provided by RefSeq, Apr 2012).
Rabbit anti Human ACTN3 antibody detects a band of 103 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
ACTN3 gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in ACTN3 results in both coding and non-coding variants. The reference genome contains the non-coding allele. The non-functional allele of ACTN3 is associated with elite athlete status (provided by RefSeq, Apr 2012).
Rabbit anti Human ACTN3 antibody detects a band of 103 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti ACTN3 detects a band of approximately 103 kDa in NIH/3T3 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid.
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography.
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose. - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Immunogen
- Synthetic peptide directed towards the N terminal region of human ACTN3
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol.
Positive Control Lysate
- Lysate Composition
- 400μg NIH/3T3 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q08043 Related reagents
- Entrez Gene
- ACTN3 Related reagents
- GO Terms
- GO:0005509 calcium ion binding
- GO:0003779 actin binding
- GO:0005829 cytosol
- GO:0005178 integrin binding
- GO:0005884 actin filament
- GO:0005925 focal adhesion
- GO:0008307 structural constituent of muscle
- GO:0042981 regulation of apoptosis
- GO:0030049 muscle filament sliding
- GO:0031143 pseudopodium
- GO:0042803 protein homodimerization activity
- GO:0048041 focal adhesion assembly
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only.
Applications of ACTN3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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