ACTN3 antibody

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Anti ACTN3 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with ACTN3 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG antibody (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 15 second exposure. Arrow points to ACTN3 (molecular weight 103 kDa).

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Rabbit anti ACTN3

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: ACTN3

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VPA00529 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human ACTN3 antibody recognizes ACTN3 also known as alpha-actinin-3, F-actin cross-linking protein, alpha-actinin skeletal muscle isoform 3.

ACTN3 gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in ACTN3 results in both coding and non-coding variants. The reference genome contains the non-coding allele. The non-functional allele of ACTN3 is associated with elite athlete status (provided by RefSeq, Apr 2012).

Rabbit anti Human ACTN3 antibody detects a band of 103 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti ACTN3 detects a band of approximately 103 kDa in NIH/3T3 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid.
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
2% Sucrose.
Immunogen: Synthetic peptide directed towards the N terminal region of human ACTN3
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: Q08043
Entrez Gene: ACTN3
GO Terms: GO:0005509 calcium ion binding; GO:0003779 actin binding; GO:0005829 cytosol; GO:0005178 integrin binding; GO:0005884 actin filament; GO:0005925 focal adhesion; GO:0008307 structural constituent of muscle; GO:0042981 regulation of apoptosis; GO:0030049 muscle filament sliding; View More GO Terms; GO:0031143 pseudopodium; GO:0042803 protein homodimerization activity; GO:0048041 focal adhesion assembly