5-Lipoxygenase (pSer523) antibody

100% Secure

Rabbit anti 5-Lipoxygenase (pSer523)

Product Type
Polyclonal Antibody
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
0.1 ml loader

Rabbit anti 5-lipoxygenase (pSer523) antibody recognizes 5-lipoxygenase (5-LO), also known as arachidonate 5-lipoxygenase, when phosphorylated at serine 523. 5-LO is an enzyme that catalyses the synthesis of leukotrienes (LTs) from arachidonic acid (a polyunsaturated fatty acid), by the insertion of an oxygen into the arachidonic backbone. 5-LO requires calcium, ATP and the 5-LO-activating protein as cofactors for full activity. Leukotrienes are synthesised and secreted by leukocytes in response to immunologic or inflammatory stimuli, such as in asthma and allergic reactions. The biological effects of LTs include leukocyte chemotaxis, pulmonary airway constriction, mucous hypersecretion, vasoconstriction and increased vascular permeability. I recent years 5-LO has also been implicated in the regulation of cell proliferation and apoptosis and has been found in a broad variety of cancer cells.

Product Details

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Primate Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to phosphorylated 5-lipoxygenase were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
10mM HEPES pH7.5, 150mM NaCl
Preservative Stabilisers
0.09%Sodium Azide
0.01%Bovine Serum Albumin
Synthetic phosphopeptide corresponding to an amino acid sequence within human 5-lipoxygenase which includes the phosphorylated residue Ser523.

Storage Information

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
12 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0005515 protein binding
GO:0004051 arachidonate 5-lipoxygenase activity
GO:0005829 cytosol
GO:0005506 iron ion binding
GO:0005641 nuclear envelope lumen
GO:0055114 oxidation-reduction process
GO:0006692 prostanoid metabolic process
GO:0016165 lipoxygenase activity
GO:0016363 nuclear matrix
GO:0019370 leukotriene biosynthetic process
GO:0031965 nuclear membrane
GO:0042446 hormone biosynthetic process
For research purposes only

Applications of 5-Lipoxygenase (pSer523) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1538 detects a doublet of approximately 80kDa in rat cortex lysate.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra