Goat anti 5-hydroxymethyluridine antibody recognises 5-hydroxymethyluridine, which is created by the oxidation of thymine by ionising radiation or reactive oxygen species. This oxidation causes changes in gene expression, mutations and chromosomal instability all of which play a role in cancer, autoimmune disease and aging. 5-hydroxymethyluridine is a potential biomarker for female breast, colon and rectal cancers, being present at elevated levels, possibly due to impaired DNA repair mechanisms.
- Target Species
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to 5-hydroxymethyluridine were raised by repeated immunisations of goats with highly purified antigen.
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 18 months from date of despatch.
- For research purposes only
Applications of 5-Hydroxymethyluridine antibody
|Application Name||Verified||Min Dilution||Max Dilution|
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|Rabbit anti Goat IgG (Fc):FITC||STAR122F||1 mg||F|
|Rabbit anti Goat IgG (Fc):HRP||STAR122P||1 mg||C E WB|
Product Specific References
References for 5-Hydroxymethyluridine antibody
Cooney, R. et al. (2001) Association of tocopherols with circulating autoantibody levels against an oxidized DNA nucleoside in humans.
Free. Radic. Biol. Med. 31:460-8.
Frenkel, K. et al. (1998) Serum autoantibodies recognizing 5-hydroxymethyl-2′-doxyuridine, an oxidized DNA base, as biomarkers of cancer risk in women.
Cancer. Epidemiol. Biomarkers Prev. 7:49–57.