5-Hydroxymethyluridine antibody

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Goat anti 5-Hydroxymethyluridine

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1257 E 0.1 ml
Goat anti 5-hydroxymethyluridine antibody recognises 5-hydroxymethyluridine, which is created by the oxidation of thymine by ionising radiation or reactive oxygen species. This oxidation causes changes in gene expression, mutations and chromosomal instability all of which play a role in cancer, autoimmune disease and aging. 5-hydroxymethyluridine is a potential biomarker for female breast, colon and rectal cancers, being present at elevated levels, possibly due to impaired DNA repair mechanisms.

Product Details

Target Species
Human
Product Form
Serum - liquid
Antiserum Preparation
Antisera to 5-hydroxymethyluridine were raised by repeated immunisations of goats with highly purified antigen.
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
5-hydroxymethyluridine.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of 5-Hydroxymethyluridine antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/25000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Product Specific References

References for 5-Hydroxymethyluridine antibody

  1. Cooney, R. et al. (2001) Association of tocopherols with circulating autoantibody levels against an oxidized DNA nucleoside in humans.
    Free. Radic. Biol. Med. 31:460-8.
  2. Frenkel, K. et al. (1998) Serum autoantibodies recognizing 5-hydroxymethyl-2′-doxyuridine, an oxidized DNA base, as biomarkers of cancer risk in women.
    Cancer. Epidemiol. Biomarkers Prev. 7:49–57.