5-HT7 Receptor antibody

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Rabbit anti Human 5-HT7 Receptor (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1344
Datasheet
P*
SDS
50 µg loader

Rabbit anti Human 5-HT& receptor antibody recognizes the N-terminal region of the serotonin (5-hydroxytryptamine, 5-HT) receptor 5-HT7, which belongs to the G-protein coupled receptor superfamily. Serotonin is a neurotransmitter and vasoactive amine which is thought to play a role in various cognitive and behavioural functions including pain, appetite, mood and sleep.

Signalling through 5-HT7 has several effects, including regulation of the circadian rhythm and mediation of smooth-muscle relaxation in the vasculature and gastrointestinal tract. Signalling though 5-HT7 has also been shown to contribute to early T-cell activation.

5-HT7 has been implicated in depression, autism, and other neuropsychiatric disorders.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to 5-HT7 receptor (NT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to the N-terminal domain of human 5-HT7 receptor, conjugated to Keyhole Limpet Hemocyanin (KLH).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P34969
Entrez Gene
HTR7
GO Terms
GO:0005515 protein binding
GO:0004993 serotonin receptor activity
GO:0005887 integral to plasma membrane
GO:0007623 circadian rhythm
GO:0008015 blood circulation
Regulatory
For research purposes only

Applications of 5-HT7 Receptor antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 10ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Brain, cortex.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
Histar Detection System STAR3000A C P 50 Tests

Product Specific References

References for 5-HT7 Receptor antibody

  1. León-ponte, M. et al. (2007) Serotonin provides an accessory signal to enhance T-cell activation by signaling through the 5-HT7 receptor.
    Blood. 109 (8): 3139-46.
  2. Soll, C. et al. (2012) Expression of serotonin receptors in human hepatocellular cancer.
    Clin Cancer Res. 18: 5902-10.