4EBP1 (pThr37) antibody

Rabbit anti 4EBP1 (pThr37)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
4EBP1 (pThr37)

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AHP2543
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Rabbit anti 4EBP1 (pThr37) antibody recognizes eukaryotic translation initiation factor 4E-binding protein 1 (4EBP1) also known as phosphorylated heat-and acid-stable protein regulated by insulin 1 (PHAS-I), when phosphorylated at threonine 37. 4EBP1 belongs to the eIF4E-binding protein family.

4EBP1, when bound to eIF4E inhibits cap-dependent translation. Phosphorylation at threonine 37 helps prime the 4EBP1 complex for further phosphorylation at multiple sites including Ser65.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to 4EBP1 (pThr37) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding threonine 37 of human 4EBP1
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/50 1/100
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
Q13541
Entrez Gene
EIF4EBP1
GO Terms
GO:0000082 G1/S transition of mitotic cell cycle
GO:0005829 cytosol
GO:0006412 translation
GO:0008286 insulin receptor signaling pathway
GO:0031929 TOR signaling cascade
GO:0045931 positive regulation of mitotic cell cycle
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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