14-3-3 Theta antibody

Rabbit anti 14-3-3 Theta

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
14-3-3 Theta

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VPA00704
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Rabbit anti Human 14-3-3 theta antibody recognizes the 14-3-3 protein theta, also known as 14-3-3 protein T-cell, 14-3-3 protein tau, 14-3-3 protein theta, or. tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide.

The YWHAQ gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. YWHAQ is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease (provided by RefSeq, Jul 2008).

Rabbit anti Human 14-3-3 theta antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Rabbit anti 14-3-3 theta detects a band of approximately 28 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
TRIS buffered glycine.
Preservative Stabilisers
0.01% Thiomersal
<50% Glycerol
Immunogen
Recombinant protein encompassing part of the middle region of human 14-3-3 theta
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P27348
Entrez Gene
YWHAQ
GO Terms
GO:0005634 nucleus
GO:0005813 centrosome
GO:0019904 protein domain specific binding
GO:0045892 negative regulation of transcription, DNA-dependent
GO:0047485 protein N-terminus binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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