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14-3-3 Sigma antibody

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Western Blot Validated
Anti 14-3-3 Sigma Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with 14-3-3 sigma antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 1 second exposure. Arrow points to 14-3-3 sigma (molecular weight 27 kDa)

Rabbit anti 14-3-3 Sigma

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VPA00717
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human 14-3-3 sigma antibody recognizes the protein 14-3-3 sigma, also known as epithelial cell marker protein 1 or stratifin.

Hypermethylation of stratifin has been linked to early stages in breast cancer. Additionally, it has been shown to promote MMP-1 expression in fibroblasts.

Rabbit anti Human 14-3-3 sigma antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Rabbit anti 14-3-3 sigma detects a band of approximately 27 kDa in A431 cell lysates
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
KLH conjugated synthetic peptide of human 14-3-3 sigma (amino acids 222 - 248)

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P31947
Entrez Gene
SFN
GO Terms
GO:0001836 release of cytochrome c from mitochondria
GO:0005615 extracellular space
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0008426 protein kinase C inhibitor activity
GO:0008630 DNA damage response, signal transduction resulting in induction of apoptosis
GO:0019904 protein domain specific binding
GO:0043154 negative regulation of caspase activity
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of 14-3-3 Sigma antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml

A Precise Solution to Reproducible Western Blots - PrecisionAb Antibodies
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