14-3-3 Gamma antibody

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Rabbit anti 14-3-3 Gamma

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00565
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human 14-3-3 gamma antibody recognizes 14-3-3 protein gamma, also known as protein kinase C inhibitor protein 1, "protein phosphatase 1, regulatory subunit 170" or tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide.

The YWHAG gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways (provided by RefSeq, Jul 2008).

Rabbit anti Human 14-3-3 gamma antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti 14-3-3 gamma detects a band of approximately 27 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
KLH conjugated synthetic peptide corresponding to amino acid 63-92 of human 14-3-3 Gamma

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P61981 Related reagents
Entrez Gene
YWHAG Related reagents
GO Terms
GO:0000086 G2/M transition of mitotic cell cycle
GO:0005829 cytosol
GO:0005080 protein kinase C binding
GO:0005159 insulin-like growth factor receptor binding
GO:0008426 protein kinase C inhibitor activity
GO:0009966 regulation of signal transduction
GO:0045664 regulation of neuron differentiation
GO:0048167 regulation of synaptic plasticity
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only.

Applications of 14-3-3 Gamma antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml

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