14-3-3 Gamma antibody

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Rabbit anti 14-3-3 Gamma

Rabbit anti-human 14-3-3 gamma antibody recognizes 14-3-3 protein gamma, also known as KCIP-1, protein kinase C inhibitor protein 1, or tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide.

14-3-3 Gamma Antibody with Control Lysate

Rabbit anti-human 14-3-3 gamma antibody recognizes 14-3-3 protein gamma, also known as KCIP-1, protein kinase C inhibitor protein 1, protein phosphatase 1, regulatory subunit 170 or tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00565 WB 100 µl
VPA00565KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human 14-3-3 gamma antibody recognizes 14-3-3 protein gamma, also known as protein kinase C inhibitor protein 1, "protein phosphatase 1, regulatory subunit 170" or tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide.

The YWHAG gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways (provided by RefSeq, Jul 2008).

Rabbit anti Human 14-3-3 gamma antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti 14-3-3 gamma detects a band of approximately 27 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide corresponding to amino acid 63-92 of human 14-3-3 Gamma
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P61981 Related reagents
Entrez Gene
YWHAG Related reagents
GO Terms
GO:0000086 G2/M transition of mitotic cell cycle
GO:0005829 cytosol
GO:0005080 protein kinase C binding
GO:0005159 insulin-like growth factor receptor binding
GO:0008426 protein kinase C inhibitor activity
GO:0009966 regulation of signal transduction
GO:0045664 regulation of neuron differentiation
GO:0048167 regulation of synaptic plasticity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of 14-3-3 Gamma antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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