14-3-3 Gamma antibody
Rabbit anti 14-3-3 Gamma
Rabbit anti-human 14-3-3 gamma antibody recognizes 14-3-3 protein gamma, also known as KCIP-1, protein kinase C inhibitor protein 1, or tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide.
14-3-3 Gamma Antibody with Control Lysate
Rabbit anti-human 14-3-3 gamma antibody recognizes 14-3-3 protein gamma, also known as KCIP-1, protein kinase C inhibitor protein 1, protein phosphatase 1, regulatory subunit 170 or tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00565 | WB | 100 µl | ||||
| VPA00565KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human 14-3-3 gamma antibody recognizes 14-3-3 protein gamma, also known as protein kinase C inhibitor protein 1, "protein phosphatase 1, regulatory subunit 170" or tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide.
The YWHAG gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways (provided by RefSeq, Jul 2008).
Rabbit anti Human 14-3-3 gamma antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The YWHAG gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways (provided by RefSeq, Jul 2008).
Rabbit anti Human 14-3-3 gamma antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti 14-3-3 gamma detects a band of approximately 27 kDa in HeLa cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid.
- Product Form
- Purified IgG - liquid.
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography.
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3).
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3).
- Immunogen
- KLH conjugated synthetic peptide corresponding to amino acid 63-92 of human 14-3-3 Gamma
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P61981 Related reagents
- Entrez Gene
- YWHAG Related reagents
- GO Terms
- GO:0000086 G2/M transition of mitotic cell cycle
- GO:0005829 cytosol
- GO:0005080 protein kinase C binding
- GO:0005159 insulin-like growth factor receptor binding
- GO:0008426 protein kinase C inhibitor activity
- GO:0009966 regulation of signal transduction
- GO:0045664 regulation of neuron differentiation
- GO:0048167 regulation of synaptic plasticity
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only.
Applications of 14-3-3 Gamma antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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