14-3-3 eta antibody

Rabbit anti 14-3-3 eta

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
14-3-3 eta

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VPA00376
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Rabbit anti Human 14-3-3 eta antibody recognizes 14-3-3 eta, also known as tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein and ETA polypeptide.

Encoded by the YWHAH gene, 14-3-3 eta belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the Mouse and bovine orthologs. YWHAH contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder (provided by RefSeq, Jun 2009).

Rabbit anti Human 14-3-3 eta antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti 14-3-3 ETA detects a band of approximately 27 kDa in PC-12 cell lysate.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide corresponding to the N-terminal region of human 14-3-3 eta
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
Q04917
Entrez Gene
YWHAH
GO Terms
GO:0005159 insulin-like growth factor receptor binding
GO:0005737 cytoplasm
GO:0006713 glucocorticoid catabolic process
GO:0006886 intracellular protein transport
GO:0016563 transcription activator activity
GO:0019899 enzyme binding
GO:0019904 protein domain specific binding
GO:0035259 glucocorticoid receptor binding
GO:0042921 glucocorticoid receptor signaling pathway
GO:0048167 regulation of synaptic plasticity
GO:0050774 negative regulation of dendrite morphogenesis
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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