14-3-3 eta antibody

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Rabbit anti 14-3-3 eta

Rabbit anti human 14-3-3 ETA antibody recognizes the 14-3-3 ETA protein, also known as YWHA1 or tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein ETA. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00376 WB 100 µl
Rabbit anti Human 14-3-3 eta antibody recognizes 14-3-3 eta, also known as tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein and ETA polypeptide.

Encoded by the YWHAH gene, 14-3-3 eta belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the Mouse and bovine orthologs. YWHAH contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder (provided by RefSeq, Jun 2009).

Rabbit anti Human 14-3-3 eta antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti 14-3-3 ETA detects a band of approximately 27 kDa in PC-12 cell lysate
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide corresponding to the N-terminal region of human 14-3-3 eta
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q04917 Related reagents
Entrez Gene
YWHAH Related reagents
GO Terms
GO:0005159 insulin-like growth factor receptor binding
GO:0005737 cytoplasm
GO:0006713 glucocorticoid catabolic process
GO:0006886 intracellular protein transport
GO:0016563 transcription activator activity
GO:0019899 enzyme binding
GO:0019904 protein domain specific binding
GO:0035259 glucocorticoid receptor binding
GO:0042921 glucocorticoid receptor signaling pathway
GO:0048167 regulation of synaptic plasticity
GO:0050774 negative regulation of dendrite morphogenesis
Acknowledgements
PrecisionAbâ„¢ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of 14-3-3 eta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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