Sheep anti Human 14-3-3 antibody
recognises the lower molecular weight subunit (26 kDa) of protein 14-3-3.
The 14-3-3 family consists of ~30 kDa proteins involved in multiple cellular processes including protein kinase signalling pathways, regulation of cell cycle progression, transcription and intracellular trafficking. Protein interactions with 14-3-3 show distinct preferences for its different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein (Bax and Jhoti 1995
- Target Species
- Product Form
- Purified Ig - liquid
- Purified Ig prepared by affinity chromatography on Protein G
- Antiserum Preparation
- Antisera to 14-3-3 were raised by repeated immunisations of sheep with highly purified antigen.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Native 14-3-3 protein, purified from pathogen-free normal human brain.
- Approx. Protein Concentrations
- Ig concentration 2.5 mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
protein domain specific binding
negative regulation of transcription, DNA-dependent
protein N-terminus binding
activation of MAPKK activity
epidermal growth factor receptor signaling pathway
Ras protein signal transduction
insulin receptor signaling pathway
induction of apoptosis by intracellular signals
activation of pro-apoptotic gene products
mRNA metabolic process
negative regulation of protein dephosphorylation
histone deacetylase binding
nerve growth factor receptor signaling pathway
perinuclear region of cytoplasm
cytoplasmic sequestering of protein
release of cytochrome c from mitochondria
protein kinase C inhibitor activity
DNA damage response, signal transduction resulting in induction of apoptosis
negative regulation of caspase activity
G2/M transition of mitotic cell cycle
protein kinase C binding
insulin-like growth factor receptor binding
regulation of signal transduction
regulation of neuron differentiation
regulation of synaptic plasticity
glucocorticoid catabolic process
intracellular protein transport
transcription activator activity
glucocorticoid receptor binding
glucocorticoid receptor signaling pathway
negative regulation of dendrite morphogenesis
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of 14-3-3 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for 14-3-3 antibody
Boston, P.F. et al. (1982) Purification, properties, and immunohistochemical localisation of human brain 14-3-3 protein.
J Neurochem. 38 (5): 1466-74.
Maronde, E. et al. (2011) Dynamics in enzymatic protein complexes offer a novel principle for the regulation of melatonin synthesis in the human pineal gland.
J Pineal Res. 51: 145-55.