14-3-3 antibody

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Sheep anti Human 14-3-3 Protein

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
7835-1009 Datasheet	WB MSDS	1 ml	Log in		Get Quote

Sheep anti Human 14-3-3 antibodyrecognises the lower molecular weight subunit (26 kDa) of protein 14-3-3.

The 14-3-3 family consists of ~30 kDa proteins involved in multiple cellular processes including protein kinase signalling pathways, regulation of cell cycle progression, transcription and intracellular trafficking. Protein interactions with 14-3-3 show distinct preferences for its different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein (Bax and Jhoti 1995)

Product Details

Target Species

Human

Product Form

Purified Ig - liquid

Preparation

Purified Ig prepared by affinity chromatography on Protein G

Antiserum Preparation

Antisera to 14-3-3 were raised by repeated immunisations of sheep with highly purified antigen.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide (NaN₃)

Immunogen

Native 14-3-3 protein, purified from pathogen-free normal human brain.

Approx. Protein Concentrations

Ig concentration 2.5 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.

More Information

UniProt: P63104 Related reagents; Q04917 Related reagents; P27348 Related reagents; P62258 Related reagents; P31947 Related reagents; P61981 Related reagents; P31946 Related reagents
Entrez Gene: YWHAQ Related reagents; YWHAB Related reagents; SFN Related reagents; YWHAG Related reagents; YWHAE Related reagents; YWHAZ Related reagents; YWHAH Related reagents
GO Terms: GO:0005634 nucleus; GO:0005813 centrosome; GO:0019904 protein domain specific binding; GO:0045892 negative regulation of transcription, DNA-dependent; GO:0047485 protein N-terminus binding; GO:0000186 activation of MAPKK activity; GO:0005829 cytosol; GO:0007173 epidermal growth factor receptor signaling pathway; GO:0007265 Ras protein signal transduction; GO:0007411 axon guidance; GO:0008286 insulin receptor signaling pathway; GO:0008629 induction of apoptosis by intracellular signals; GO:0008633 activation of pro-apoptotic gene products; GO:0016071 mRNA metabolic process; GO:0035308 negative regulation of protein dephosphorylation; GO:0042470 melanosome; GO:0042826 histone deacetylase binding; GO:0050815 phosphoserine binding; GO:0048011 nerve growth factor receptor signaling pathway; GO:0048471 perinuclear region of cytoplasm; GO:0051220 cytoplasmic sequestering of protein; GO:0001836 release of cytochrome c from mitochondria; GO:0005615 extracellular space; GO:0005737 cytoplasm; GO:0008426 protein kinase C inhibitor activity; GO:0008630 DNA damage response, signal transduction resulting in induction of apoptosis; GO:0043154 negative regulation of caspase activity; GO:0000086 G2/M transition of mitotic cell cycle; GO:0005080 protein kinase C binding; GO:0005159 insulin-like growth factor receptor binding; GO:0009966 regulation of signal transduction; GO:0045664 regulation of neuron differentiation; GO:0048167 regulation of synaptic plasticity; GO:0006713 glucocorticoid catabolic process; GO:0006886 intracellular protein transport; GO:0016563 transcription activator activity; GO:0019899 enzyme binding; GO:0035259 glucocorticoid receptor binding; GO:0042921 glucocorticoid receptor signaling pathway; GO:0050774 negative regulation of dendrite morphogenesis
Regulatory: For research purposes only

Applications of 14-3-3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/10	1/50

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Rabbit anti Sheep IgG (H/L):Biotin	5184-2304	C E F WB	1.5 ml	Log in	Get Quote
Donkey anti Sheep/Goat IgG:DyLight®488	STAR88D488GA	F IF	0.1 mg	Log in	Get Quote
Donkey anti Sheep/Goat IgG:FITC	STAR88F	C	1 mg	Log in	Get Quote
Donkey anti Sheep/Goat IgG:HRP	STAR88P	C E P WB	1 ml	Log in	Get Quote

Product Specific References

References for 14-3-3 antibody

Boston, P.F. et al. (1982) Purification, properties, and immunohistochemical localisation of human brain 14-3-3 protein.
J Neurochem. 38 (5): 1466-74.
Maronde, E. et al. (2011) Dynamics in enzymatic protein complexes offer a novel principle for the regulation of melatonin synthesis in the human pineal gland.
J Pineal Res. 51: 145-55.

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