14-3-3 antibody

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Sheep anti Human 14-3-3 Protein

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
7835-1009 WB 1 ml
Sheep anti Human 14-3-3 antibodyrecognises the lower molecular weight subunit (26 kDa) of protein 14-3-3.

The 14-3-3 family consists of ~30 kDa proteins involved in multiple cellular processes including protein kinase signalling pathways, regulation of cell cycle progression, transcription and intracellular trafficking. Protein interactions with 14-3-3 show distinct preferences for its different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein (Bax and Jhoti 1995)

Product Details

Target Species
Human
Product Form
Purified Ig - liquid
Preparation
Purified Ig prepared by affinity chromatography on Protein G
Antiserum Preparation
Antisera to 14-3-3 were raised by repeated immunisations of sheep with highly purified antigen.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Native 14-3-3 protein, purified from pathogen-free normal human brain.
Approx. Protein Concentrations
Ig concentration 2.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P63104 Related reagents
Q04917 Related reagents
P27348 Related reagents
P62258 Related reagents
P31947 Related reagents
P61981 Related reagents
P31946 Related reagents
Entrez Gene
YWHAQ Related reagents
YWHAB Related reagents
SFN Related reagents
YWHAG Related reagents
YWHAE Related reagents
YWHAZ Related reagents
YWHAH Related reagents
GO Terms
GO:0005634 nucleus
GO:0005813 centrosome
GO:0019904 protein domain specific binding
GO:0045892 negative regulation of transcription, DNA-dependent
GO:0047485 protein N-terminus binding
GO:0000186 activation of MAPKK activity
GO:0005829 cytosol
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007265 Ras protein signal transduction
GO:0007411 axon guidance
GO:0008286 insulin receptor signaling pathway
GO:0008629 induction of apoptosis by intracellular signals
GO:0008633 activation of pro-apoptotic gene products
GO:0016071 mRNA metabolic process
GO:0035308 negative regulation of protein dephosphorylation
GO:0042470 melanosome
GO:0042826 histone deacetylase binding
GO:0050815 phosphoserine binding
GO:0048011 nerve growth factor receptor signaling pathway
GO:0048471 perinuclear region of cytoplasm
GO:0051220 cytoplasmic sequestering of protein
GO:0001836 release of cytochrome c from mitochondria
GO:0005615 extracellular space
GO:0005737 cytoplasm
GO:0008426 protein kinase C inhibitor activity
GO:0008630 DNA damage response, signal transduction resulting in induction of apoptosis
GO:0043154 negative regulation of caspase activity
GO:0000086 G2/M transition of mitotic cell cycle
GO:0005080 protein kinase C binding
GO:0005159 insulin-like growth factor receptor binding
GO:0009966 regulation of signal transduction
GO:0045664 regulation of neuron differentiation
GO:0048167 regulation of synaptic plasticity
GO:0006713 glucocorticoid catabolic process
GO:0006886 intracellular protein transport
GO:0016563 transcription activator activity
GO:0019899 enzyme binding
GO:0035259 glucocorticoid receptor binding
GO:0042921 glucocorticoid receptor signaling pathway
GO:0050774 negative regulation of dendrite morphogenesis
Regulatory
For research purposes only

Applications of 14-3-3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/10 1/50
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti 14-3-3 Isoform Panel PAN017 25 µl X 7 E* P* WB*

Product Specific References

References for 14-3-3 antibody

  1. Boston, P.F. et al. (1982) Purification, properties, and immunohistochemical localisation of human brain 14-3-3 protein.
    J Neurochem. 38 (5): 1466-74.
  2. Maronde, E. et al. (2011) Dynamics in enzymatic protein complexes offer a novel principle for the regulation of melatonin synthesis in the human pineal gland.
    J Pineal Res. 51: 145-55.