14-3-3 antibody
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Sheep anti Human 14-3-3 antibodyrecognises the lower molecular weight subunit (26 kDa) of protein 14-3-3.
The 14-3-3 family consists of ~30 kDa proteins involved in multiple cellular processes including protein kinase signalling pathways, regulation of cell cycle progression, transcription and intracellular trafficking. Protein interactions with 14-3-3 show distinct preferences for its different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein (Bax and Jhoti 1995)
The 14-3-3 family consists of ~30 kDa proteins involved in multiple cellular processes including protein kinase signalling pathways, regulation of cell cycle progression, transcription and intracellular trafficking. Protein interactions with 14-3-3 show distinct preferences for its different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein (Bax and Jhoti 1995)
Product Details
- Target Species
- Human
- Product Form
- Purified Ig - liquid
- Preparation
- Purified Ig prepared by affinity chromatography on Protein G
- Antiserum Preparation
- Antisera to 14-3-3 were raised by repeated immunisations of sheep with highly purified antigen.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Immunogen
- Native 14-3-3 protein, purified from pathogen-free normal human brain.
- Approx. Protein Concentrations
- Ig concentration 2.5 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P63104 Related reagents
- Q04917 Related reagents
- P27348 Related reagents
- P62258 Related reagents
- P31947 Related reagents
- P61981 Related reagents
- P31946 Related reagents
- Entrez Gene
- YWHAQ Related reagents
- YWHAB Related reagents
- SFN Related reagents
- YWHAG Related reagents
- YWHAE Related reagents
- YWHAZ Related reagents
- YWHAH Related reagents
- GO Terms
- GO:0005634 nucleus
- GO:0005813 centrosome
- GO:0019904 protein domain specific binding
- GO:0045892 negative regulation of transcription, DNA-dependent
- GO:0047485 protein N-terminus binding
- GO:0000186 activation of MAPKK activity
- GO:0005829 cytosol
- GO:0007173 epidermal growth factor receptor signaling pathway
- GO:0007265 Ras protein signal transduction
- GO:0007411 axon guidance
- GO:0008286 insulin receptor signaling pathway
- GO:0008629 induction of apoptosis by intracellular signals
- GO:0008633 activation of pro-apoptotic gene products
- GO:0016071 mRNA metabolic process
- GO:0035308 negative regulation of protein dephosphorylation
- GO:0042470 melanosome
- GO:0042826 histone deacetylase binding
- GO:0050815 phosphoserine binding
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0048471 perinuclear region of cytoplasm
- GO:0051220 cytoplasmic sequestering of protein
- GO:0001836 release of cytochrome c from mitochondria
- GO:0005615 extracellular space
- GO:0005737 cytoplasm
- GO:0008426 protein kinase C inhibitor activity
- GO:0008630 DNA damage response, signal transduction resulting in induction of apoptosis
- GO:0043154 negative regulation of caspase activity
- GO:0000086 G2/M transition of mitotic cell cycle
- GO:0005080 protein kinase C binding
- GO:0005159 insulin-like growth factor receptor binding
- GO:0009966 regulation of signal transduction
- GO:0045664 regulation of neuron differentiation
- GO:0048167 regulation of synaptic plasticity
- GO:0006713 glucocorticoid catabolic process
- GO:0006886 intracellular protein transport
- GO:0016563 transcription activator activity
- GO:0019899 enzyme binding
- GO:0035259 glucocorticoid receptor binding
- GO:0042921 glucocorticoid receptor signaling pathway
- GO:0050774 negative regulation of dendrite morphogenesis
- Regulatory
- For research purposes only
Applications of 14-3-3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/10 | 1/50 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | 1.5 ml | C E F WB | ||
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | 0.1 mg | F IF | ||
| Donkey anti Sheep/Goat IgG:DyLight®549 | STAR88D549GA | 0.1 mg | F IF | ||
| Donkey anti Sheep/Goat IgG:DyLight®649 | STAR88D649GA | 0.1 mg | F IF | ||
| Donkey anti Sheep/Goat IgG:FITC | STAR88F | 1 mg | C | ||
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti 14-3-3 Isoform Panel | PAN017 | 25 µl X 7 | E* P* WB* |
Product Specific References
References for 14-3-3 antibody
-
Boston, P.F. et al. (1982) Purification, properties, and immunohistochemical localisation of human brain 14-3-3 protein.
J Neurochem. 38 (5): 1466-74. -
Maronde, E. et al. (2011) Dynamics in enzymatic protein complexes offer a novel principle for the regulation of melatonin synthesis in the human pineal gland.
J Pineal Res. 51: 145-55.
