Rabbit anti Histone acetylated H3Histone antibody was generated using a peptide corresponding to H3 acetylated at lysine 14 and recognizes all or most acetylated H3 isoforms.
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to acetylated histone H3 were raised by repeated immunisation of rabbits with highly purified antigen.
- Preservative Stabilisers
- Ovalbumin-conjugated peptide: NKSTGGAcKAPRKYCc
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Histone H3 Acetylated antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Application Based External Images
Immunohistology - Frozen
Product Specific References
References for Histone H3 Acetylated antibody
Belyaev, N. et al. (1996) Differential underacetylation of histones H2A, H3 and H4 on the inactive X chromosome in human female cells.
Hum Genet. 97 (5): 573-8.
Tsaprouni, L.G. et al. (2011) Differential patterns of histone acetylation in inflammatory bowel diseases.
J Inflamm (Lond). 8: 1.
Foy, R.L. et al. (2008) Role of Jade-1 in the histone acetyltransferase (HAT) HBO1 complex.
J Biol Chem. 283: 28817-26.
Havasi, A. et al. (2013) Histone Acetyl Transferase (HAT) HBO1 and JADE1 in Epithelial Cell Regeneration.
Am J Pathol. 182: 152-62.
Epping MT et al. (2009) UNC45A confers resistance to histone deacetylase inhibitors and retinoic acid.
Mol Cancer Res. 7 (11): 1861-70.
How to Use the Spectraviewer?
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra