Histone H3 Acetylated antibody
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Rabbit anti Histone acetylated H3Histone antibody was generated using a peptide corresponding to H3 acetylated at lysine 14 and recognizes all or most acetylated H3 isoforms.
Product Details
- Species Cross-Reactivity
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Target Species Cross Reactivity Mammals Yeast Amphibia - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to acetylated histone H3 were raised by repeated immunisation of rabbits with highly purified antigen.
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Ovalbumin-conjugated peptide: NKSTGGAcKAPRKYCc
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q6NXT2
- Entrez Gene
- H3F3C
- GO Terms
- GO:0000786 nucleosome
- GO:0003677 DNA binding
- GO:0006334 nucleosome assembly
- GO:0005634 nucleus
- Regulatory
- For research purposes only
Applications of Histone H3 Acetylated antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/100 | ||
| Immunofluorescence | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for Histone H3 Acetylated antibody
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Belyaev, N. et al. (1996) Differential underacetylation of histones H2A, H3 and H4 on the inactive X chromosome in human female cells.
Hum Genet. 97 (5): 573-8. -
Tsaprouni, L.G. et al. (2011) Differential patterns of histone acetylation in inflammatory bowel diseases.
J Inflamm (Lond). 8: 1. -
Foy, R.L. et al. (2008) Role of Jade-1 in the histone acetyltransferase (HAT) HBO1 complex.
J Biol Chem. 283: 28817-26. -
Havasi, A. et al. (2013) Histone Acetyl Transferase (HAT) HBO1 and JADE1 in Epithelial Cell Regeneration.
Am J Pathol. 182: 152-62. -
Epping MT et al. (2009) UNC45A confers resistance to histone deacetylase inhibitors and retinoic acid.
Mol Cancer Res. 7 (11): 1861-70.
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