Histone H3 Acetylated antibody
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Rabbit anti Histone acetylated H3Histone antibody was generated using a peptide corresponding to H3 acetylated at lysine 14 and recognizes all or most acetylated H3 isoforms.
Product Details
- Species Cross-Reactivity
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Target Species Cross Reactivity Mammals Yeast Amphibia - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to acetylated histone H3 were raised by repeated immunisation of rabbits with highly purified antigen.
- Preservative Stabilisers
0.02% Sodium Azide - Immunogen
- Ovalbumin-conjugated peptide: NKSTGGAcKAPRKYCc
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q6NXT2
- Entrez Gene
- H3F3C
- GO Terms
- GO:0000786 nucleosome
- GO:0003677 DNA binding
- GO:0006334 nucleosome assembly
- GO:0005634 nucleus
- Regulatory
- For research purposes only
Applications of Histone H3 Acetylated antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/100 | ||
| Immunofluorescence | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for Histone H3 Acetylated antibody
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Belyaev, N. et al. (1996) Differential underacetylation of histones H2A, H3 and H4 on the inactive X chromosome in human female cells.
Hum Genet. 97 (5): 573-8. -
Tsaprouni, L.G. et al. (2011) Differential patterns of histone acetylation in inflammatory bowel diseases.
J Inflamm (Lond). 8: 1. -
Foy, R.L. et al. (2008) Role of Jade-1 in the histone acetyltransferase (HAT) HBO1 complex.
J Biol Chem. 283: 28817-26. -
Havasi, A. et al. (2013) Histone Acetyl Transferase (HAT) HBO1 and JADE1 in Epithelial Cell Regeneration.
Am J Pathol. 182: 152-62. -
Epping MT et al. (2009) UNC45A confers resistance to histone deacetylase inhibitors and retinoic acid.
Mol Cancer Res. 7 (11): 1861-70.
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