Green Fluorescent Protein antibody

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Rabbit anti Green Fluorescent Protein

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2984
Datasheet
WB
MSDS
50 µg loader

Rabbit anti GFP antibody recognizes green fluorescent protein (GFP) and its variants. GFP is a ~27 kDa protein derived from the jellyfish Aequorea victoria. GFP fluoresces green (509 nm) when excited by blue light (395 nm), and is commonly used as a marker of gene expression. Rabbit anti GFP antibody is known to cross-react with enhanced green fluorescent protein (EGFP), yellow fluorescent protein (YFP) and red fluorescent protein (RFP).

Product Details

Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to green fluorescent protein (GFP) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
30% Glycerol
0.5% Bovine Serum Albumin
0.01% Thiomersal
Immunogen
E. coli expressed recombinant EGFP
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Acknowledgements
ProclinTM 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow
Regulatory
For research purposes only

Applications of Green Fluorescent Protein antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 0.5 4.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti GFP antibody recognizes a band of approximately 30-37 kDa on recombinant enhanced green fluorescent protein (EGFP).
Rabbit anti GFP antibody recognizes a band of approximately 32-34 kDa on recombinant red fluorescent protein (RFP).
Rabbit anti GFP antibody recognizes a band of approximately 32-34 kDa on recombinant yellow fluorescent protein (YFP).

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B C E P WB 1 ml loader
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg loader
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg loader
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti mCherry AHP2326 WB 50 µg loader
Rabbit anti Blue Fluorescent Protein AHP2985 WB 50 µg loader
Rabbit anti Cyan Fluorescent Protein AHP2986 WB 50 µg loader
Rabbit anti Red Fluorescent Protein AHP2987 WB 50 µg loader
Mouse anti mCherry MCA6020 WB 50 µg loader