Green Fluorescent Protein antibody

Rabbit anti Green Fluorescent Protein

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Green Fluorescent Protein

Product Code Applications Pack Size List Price Your Price Qty
AHP2984
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 50 µg loader
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loader
Search for Batch Specific Datasheets

Rabbit anti GFP antibody recognizes green fluorescent protein (GFP) and its variants. GFP is a ~27 kDa protein derived from the jellyfish Aequorea victoria. GFP fluoresces green (509 nm) when excited by blue light (395 nm), and is commonly used as a marker of gene expression. Rabbit anti GFP antibody is known to cross-react with enhanced green fluorescent protein (EGFP), yellow fluorescent protein (YFP) and red fluorescent protein (RFP).

Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to green fluorescent protein (GFP) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
30% Glycerol
0.5% Bovine Serum Albumin
0.03% Proclin 300
Immunogen
E. coli expressed recombinant EGFP
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
Proclin 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow

Store at -20°C
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 0.5 4.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti GFP antibody recognizes a band of approximately 30-37 kDa on recombinant enhanced green fluorescent protein (EGFP).
Rabbit anti GFP antibody recognizes a band of approximately 32-34 kDa on recombinant red fluorescent protein (RFP).
Rabbit anti GFP antibody recognizes a band of approximately 32-34 kDa on recombinant yellow fluorescent protein (YFP).

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Synonyms
Gfp
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"