Tyrosinase antibody

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Sheep anti Mushroom Tyrosinase

Sheep anti Mushroom Tyrosinase

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
9344-9979G E WB 1 ml
9344-9979 E WB 0.2 ml
Sheep anti Mushroom tyrosinase antibody recognizes mushroom tyrosinase, also known as 5,6-dihydroxyindole-2-carboxylic acid oxidase, DHICA oxidase, Glycoprotein 75, Melanoma antigen gp75, Tyrosinase-related protein 1 or TRP-1. Tyrosinase is widely distributed in both antmals and plants, it is a copper containing oxidase involved in the biosynthesis of melanin in mammals and is involved in fruit browning reactions (Thalmann and Lötzbeyer 2002).

In man, mutations in the TYRP1 gene can lead to the development of Albinism, oculocutaneous, 3 (OCA3), a condition characterized by reduced deposition of melanin in the skin, hair and eyes.

Product Details

Target Species
Fungal
Product Form
Purified IgG - liquid
Product Form
Serum - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified native mushroom tyrosinase
Approx. Protein Concentrations
IgG concentration 5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
O42713 Related reagents
GO Terms
GO:0004503 monophenol monooxygenase activity
GO:0046872 metal ion binding
GO:0055114 oxidation-reduction process
GO:0042438 melanin biosynthetic process
Regulatory
For research purposes only

Applications of Tyrosinase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500 1/5000
Western Blotting
ELISA
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
ELISA
In a simple ELISA with 1 µg of antigen coated per well, the 50% OD was observed at 1/20,000. Under the same conditions, the end point was observed at >1/400,000. Alkaline phosphatase conjugated anti-sheep was used for detection. All incubations at 18ºC using pNPP substrate, with OD measurements at 405 nm.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Product Specific References

References for Tyrosinase antibody

  1. Lezzi, C. et al. (2012) Production of recombinant Agaricus bisporus tyrosinase in Saccharomyces cerevisiae cells.
    J Ind Microbiol Biotechnol. 39 (12): 1875-80.