Acetylcholinesterase antibody

Rabbit anti Electric Eel Acetylcholinesterase

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Acetylcholinesterase

Product Code Applications Pack Size List Price Your Price Qty
0200-0042
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 1 ml loader
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Rabbit anti Electric Eel Acetylcholinesterase antibody recognizes actylcholinesterase from Electrophorus electricus (electric eel).
Acetylcholinesterase exists as a homotetramer composed of 4 70kDa subunits. Each subunit contains 1 enzymatically active site.
Acetylcholinesterase catalyses the hydrolysis of acetylcholine to acetate and choline.

Target Species
Fish
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Highly purified acetylcholine esterase from Electrophorus electricus. EC no. 3.1.1.7
Approx. Protein Concentrations
IgG concentration 5.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/6K 1/24K
Western Blotting 1/10 1/50
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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References for Acetylcholinesterase antibody

  1. Mappouras, D.G. et al. (1995) Antibodies to acetylcholinesterase cross-reacting with thyroglobulin in myasthenia gravis and Graves's disease.
    Clin Exp Immunol. 100: 336-43.

RRID
AB_619316
UniProt
O42275
GO Terms
GO:0003990 acetylcholinesterase activity
GO:0004104 cholinesterase activity
GO:0005576 extracellular region
GO:0030054 cell junction
GO:0042135 neurotransmitter catabolic process
GO:0045202 synapse
View more products with ACETYLCHOLINESTERASE specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Fish Products