Thyroid Stimulating Hormone antibody

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Rabbit anti Dog Thyroid Stimulating Hormone

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
8921-5007 C E 50 µl
Rabbit anti Dog thyroid stimulating hormone polyclonal antibody was raised against highly purified intact canine thyroid stimulating hormone (TSH) and is specific for canine TSH. Rabbit anti Dog thyroid stimulating hormone exhibits less than 0.1% cross reactivity with the other major canine glycoprotein hormones; growth hormone (GH), luteinizing hormone (LH) and follicle stimulating hormone (FSH) or prolactin using an iodinated hormone tracer assay. This strongly indicates that the anti thyroid stimulating hormone antibody is primarily reactive with the TSHβ chain although it may also have reactivity with β chain conformational epitopes dependent on the presence of the common α chain shared with the other glycoprotein hormones.

TSH, also known as thyrotropin is synthesized in, and secreted from thyrotropes within the anterior pituitary gland, under the influence of secreted thyrotropin releasing hormone from the hypothalamus. TSH is composed of, as are the other major glycoprotein hormones, a common α chain non-covalently linked to a unique β chain responsible for conferring the structural and biological identity of the individual hormone. TSH binds to the TSH receptor (TSHR) in the thyroid gland. TSHR has also bee detected in other cell types including adipocytes, neuronal cells and gastric mucosa.

Defects in the TSHβ subunit can lead to hypothyroidism, either through the lack of secreted hormone or secretion of TSH lacking full biological activity (Szkudlinski et al. 2002).

Product Details

Target Species
Dog
Product Form
Ig fraction - lyophilised
Reconstitution
Reconstitute with sterile distilled water. For long term storage the addition of a preservative is recommended.
Preparation
Ig fraction prepared by ammonium sulphate precipitation
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Purified canine TSH.

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P54828 Related reagents
Q9XSW8 Related reagents
Entrez Gene
TSHB Related reagents
CGA Related reagents
GO Terms
GO:0005576 extracellular region
GO:0005179 hormone activity
Regulatory
For research purposes only

Applications of Thyroid Stimulating Hormone antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Product Specific References

Further Reading

  1. Szkudlinski, M.W. et al. (2002) Thyroid-stimulating hormone and thyroid-stimulating hormone receptor structure-function relationships.
    Physiol Rev. 82: 473-502.