IgG antibody

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Sheep anti Dog IgG:Alk. Phos.

Sheep anti Dog IgG:HRP

Sheep anti Dog IgG

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAI32AB E 0.5 mg
AAI32P E 1 mg
AAI32 C E ID WB 1 mg
Sheep anti Dog IgG polyclonal antibody recognizes canine IgG and shows no cross reactivity with other canine immunoglobulin classes in immunoelectrophoresis or ELISA. Sheep anti Dog IgG may cross react with IgG from other species. Cross reactivity has been demonstrated with the Raccoon Dog (Nyctereutes procyonoides), an early canid species (Sukura et al. 2002) .

Product Details

Target Species
Dog
Species Cross-Reactivity
Target SpeciesCross Reactivity
Raccoon dog
Sidestriped Jackal
Black-backed Jackal
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to canine IgG were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
50mM HEPES, 0.1M NaCl, 1mM MgCl2, 0.1mM ZnCl2
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.2%Bovine Serum Albumin
Preservative Stabilisers
0.1% Proclin™ 300
0.2% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Purified canine IgG.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC.
DO NOT FREEZE.

This product should be stored undiluted.
Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Shelf Life
12 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

Acknowledgements
Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/10000
ELISA 1/10,000 1/100,000
ELISA 1/100 1/30000
Immunodiffusion
Immunohistology - Frozen 1/200 1/2000
Western Blotting 1/1000 1/30000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Application Based External Images

ELISA

Product Specific References

References for IgG antibody

  1. Benyacoub, J. et al. (2003) Supplementation of food with Enterococcus faecium (SF68) stimulates immune functions in young dogs.
    J. Nutr. 133: 1158-1162.
  2. Xu, L. et al. (2007) Immune response after neonatal transfer of a human factor IX-expressing retroviral vector in dogs, cats, and mice.
    Thromb Res. 120: 269-80.
  3. Bird, R.C. et al. (2011) An autologous dendritic cell canine mammary tumor hybrid-cell fusion vaccine.
    Cancer Immunol Immunother. 60: 87-97.
  4. Miller, A.G. (2012) Antiphospholipid antibodies in dogs with immune mediated hemolytic anemia, spontaneous thrombosis, and hyperadrenocorticism.
    J Vet Intern Med. 26: 614-23.
  5. Rinkardt, N.E. et al. (1999) The effects of prednisone and azathioprine on circulating immunoglobulin levels and lymphocyte subpopulations in normal dogs.
    Can J Vet Res. 63: 18-24.
  6. Zhang, J. et al. (2004) Neonatal gene transfer with a retroviral vector results in tolerance to human factor IX in mice and dogs.
    Blood. 103: 143-51.
  7. Sukura, A. et al. (2002) Trichinella nativa and T. spiralis induce distinguishable histopathologic and humoral responses in the raccoon dog (Nyctereutes procyonoides).
    Vet Pathol. 39: 257-65.
  8. Carli, E. et al. (2009) Detection of erythrocyte binding IgM and IgG by flow cytometry in sick dogs with Babesia canis canis or Babesia canis vogeli infection.
    Vet Parasitol. 162: 51-7.
  9. Tan, E. et al. (2012) Potentially antigenic RBC membrane proteins in dogs with primary immune-mediated hemolytic anemia.
    Vet Clin Pathol. 41: 45-55.
  10. Wieland, B. et al. (2012) Prevalence of perinuclear antineutrophilic cytoplasmic autoantibodies in serum of healthy Soft Coated Wheaten Terriers in the United Kingdom.
    Am J Vet Res. 73: 404-8.
  11. Xu, L. et al. (2003) Neonatal or hepatocyte growth factor-potentiated adult gene therapy with a retroviral vector results in therapeutic levels of canine factor IX for hemophilia B.
    Blood. 101: 3924-32.
  12. Spencer, J.A. et al. (1999) Presence of antibodies to canine distemper virus, canine parvovirus and canine adenovirus type 1 in free-ranging jackals (Canis adustus and Canis mesomelas) in Zimbabwe.
    Onderstepoort J Vet Res. 66: 251-3.
  13. Shapiro, A.J. et al. (2016) Seroprevalence of Coxiella burnetii in Australian dogs.
    Zoonoses Public Health. 63 (6): 458-66.