Cyan Fluorescent Protein antibody
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WB ResetRabbit anti Cyan Fluorescent Protein
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Cyan Fluorescent Protein
Antibody Quality and Performance Guarantee
| Rabbit anti CFP antibody recognizes the cyan fluorescent protein (CFP), a monomeric engineered derivate of green fluorescent protein (GFP) isolated from the jellyfish Aequorea victoria. CFP is a ~31 kDa protein that is optimally excited at a wavelength of 439 nm and emits light at 476 nm (Day and Davidson 2009). In FRET (Fluorescence/Förster Resonance Energy Transfer) experiments, CFP is often used in combination with YFP (yellow fluorescent protein) (Karpova and McNally 2006). |
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to cyan fluorescent protein (CFP) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 30% Glycerol
0.5% Bovine Serum Albumin
0.01% Thiomersal - Immunogen
- E. coli expressed recombinant cyan fluorescent protein
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
0.5 | 4.0 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti CFP antibody recognizes a band of approximately 31 kDa on recombinant CFP. The observed band size is approximately 35 kDa. This antibody has minimum cross-reactivity with Yellow Fluorescent Protein.
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Further Reading
-
Day, R.N. & Davidson, M.W. (2009) The fluorescent protein palette: tools for cellular imaging.
Chem Soc Rev. 38 (10): 2887-921. -
Karpova, T. & McNally, J.G. (2006) Detecting protein-protein interactions with CFP-YFP FRET by acceptor photobleaching.
Curr Protoc Cytom. Chapter 12: Unit12.7.
- Synonyms
- Cfp
AHP2986
151202
159904
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