Cyan Fluorescent Protein antibody

Rabbit anti Cyan Fluorescent Protein

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Cyan Fluorescent Protein

Product Code Applications Pack Size List Price Your Price Qty
AHP2986
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 50 µg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rabbit anti CFP antibody recognizes the cyan fluorescent protein (CFP), a monomeric engineered derivate of green fluorescent protein (GFP) isolated from the jellyfish Aequorea victoria. CFP is a ~31 kDa protein that is optimally excited at a wavelength of 439 nm and emits light at 476 nm (Day and Davidson 2009). In FRET (Fluorescence/Förster Resonance Energy Transfer) experiments, CFP is often used in combination with YFP (yellow fluorescent protein) (Karpova and McNally 2006).

Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to cyan fluorescent protein (CFP) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
30% Glycerol
0.5% Bovine Serum Albumin
0.01% Thiomersal
Immunogen
E. coli expressed recombinant cyan fluorescent protein
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 0.5 4.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti CFP antibody recognizes a band of approximately 31 kDa on recombinant CFP. The observed band size is approximately 35 kDa. This antibody has minimum cross-reactivity with Yellow Fluorescent Protein.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (Fc):Biotin
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (Fc):FITC
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (Fc):HRP
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (H/L):HRP
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
List Price Your Price
loader
Description Sheep anti Rabbit IgG:FITC
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
List Price Your Price
loader
Description Sheep anti Rabbit IgG:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti mCherry AHP2326 WB 50 µg loader
List Price Your Price
loader
Description Rabbit anti mCherry
Rabbit anti Green Fluorescent Protein AHP2984 WB 50 µg loader
List Price Your Price
loader
Description Rabbit anti Green Fluorescent Protein
Rabbit anti Blue Fluorescent Protein AHP2985 WB 50 µg loader
List Price Your Price
loader
Description Rabbit anti Blue Fluorescent Protein
Rabbit anti Red Fluorescent Protein AHP2987 WB 50 µg loader
List Price Your Price
loader
Description Rabbit anti Red Fluorescent Protein
Mouse anti mCherry MCA6020 WB 50 µg loader
List Price Your Price
loader
Description Mouse anti mCherry

Further Reading

  1. Day, R.N. & Davidson, M.W. (2009) The fluorescent protein palette: tools for cellular imaging.
    Chem Soc Rev. 38 (10): 2887-921.
  2. Karpova, T. & McNally, J.G. (2006) Detecting protein-protein interactions with CFP-YFP FRET by acceptor photobleaching.
    Curr Protoc Cytom. Chapter 12: Unit12.7.

Synonyms
Cfp
Request a different product with this specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"