Cyan Fluorescent Protein antibody
Product Details
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to cyan fluorescent protein (CFP) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 30% Glycerol
0.5% Bovine Serum Albumin
0.01% Thiomersal - Immunogen
- E. coli expressed recombinant cyan fluorescent protein
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Cyan Fluorescent Protein antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Western Blotting | 0.5 | 4.0 ug/ml |
- Western Blotting
- Rabbit anti CFP antibody recognizes a band of approximately 31 kDa on recombinant CFP. The observed band size is approximately 35 kDa. This antibody has minimum cross-reactivity with Yellow Fluorescent Protein.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rabbit anti mCherry | AHP2326 | WB | 50 µg |
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Rabbit anti Green Fluorescent Protein | AHP2984 | WB | 50 µg |
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Rabbit anti Blue Fluorescent Protein | AHP2985 | WB | 50 µg |
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Rabbit anti Red Fluorescent Protein | AHP2987 | WB | 50 µg |
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Mouse anti mCherry | MCA6020 | WB | 50 µg |
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Product Specific References
Further Reading
-
Day, R.N. & Davidson, M.W. (2009) The fluorescent protein palette: tools for cellular imaging.
Chem Soc Rev. 38 (10): 2887-921. -
Karpova, T. & McNally, J.G. (2006) Detecting protein-protein interactions with CFP-YFP FRET by acceptor photobleaching.
Curr Protoc Cytom. Chapter 12: Unit12.7.
Fluorescent Spectraviewer
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How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra