Cyan Fluorescent Protein antibody
Rabbit anti CFP antibody recognizes the cyan fluorescent protein (CFP), a monomeric engineered derivate of green fluorescent protein (GFP) isolated from the jellyfish Aequorea victoria. CFP is a ~31 kDa protein that is optimally excited at a wavelength of 439 nm and emits light at 476 nm (Day and Davidson 2009). In FRET (Fluorescence/Förster Resonance Energy Transfer) experiments, CFP is often used in combination with YFP (yellow fluorescent protein) (Karpova and McNally 2006).
Product Details
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to cyan fluorescent protein (CFP) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 30% Glycerol
0.5% Bovine Serum Albumin
0.01% Thiomersal - Immunogen
- E. coli expressed recombinant cyan fluorescent protein
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Cyan Fluorescent Protein antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 0.5 | 4.0 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti CFP antibody recognizes a band of approximately 31 kDa on recombinant CFP. The observed band size is approximately 35 kDa. This antibody has minimum cross-reactivity with Yellow Fluorescent Protein.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti mCherry | AHP2326 | 50 µg | WB | ||
| Rabbit anti Green Fluorescent Protein | AHP2984 | 50 µg | WB | ||
| Rabbit anti Blue Fluorescent Protein | AHP2985 | 50 µg | WB | ||
| Rabbit anti Red Fluorescent Protein | AHP2987 | 50 µg | WB | ||
| Mouse anti mCherry | MCA6020 | 50 µg | WB |
Product Specific References
Further Reading
-
Day, R.N. & Davidson, M.W. (2009) The fluorescent protein palette: tools for cellular imaging.
Chem Soc Rev. 38 (10): 2887-921. -
Karpova, T. & McNally, J.G. (2006) Detecting protein-protein interactions with CFP-YFP FRET by acceptor photobleaching.
Curr Protoc Cytom. Chapter 12: Unit12.7.
