Ubiquitin antibody

Sheep anti Bovine Ubiquitin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Ubiquitin

Product Code Applications Pack Size List Price Your Price Qty
9400-0409G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 1 ml
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Search for Batch Specific Datasheets

Sheep anti Bovine ubiquitin antibody recognizes ubiquitin, a 8.6 kDa cytoplasmic and nuclear protein highly conserved across species.

Ubiquitin functions as a protein modifier which covalently attaches to lysines as either a monomer or as a lysine-linked polymer. Attachment as a Lys-48 polymer usually leads to the target protein undergoing proteolytic degradation. Attachment as a monomer does not result in degradation and may be linked to functions including maintenance of chromatin structure, regulation of gene expression, stress response and DNA repair.

Ubiquitin can be detected in both normal and diseased brain tissues including the neurofibrillary tangles of Alzheimers disease, Lewy bodies in Parkinsons disease and Pick bodies in Pick’s disease.

Target Species
Bovine
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Native ubiquitin, purified from bovine erythrocytes to a purity ≥ 90% by SDS-PAGE.
Approx. Protein Concentrations
IgG concentration 5.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
ELISA
Sheep anti Bovine Ubiquitin antibody may be used in an indirect ELISA with native bovine ubiquitin (9400-1502) as the standard.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 C E F WB 1.5 ml loader
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Description Rabbit anti Sheep IgG (H/L):Biotin
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA F IF 0.1 mg loader
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Description Donkey anti Sheep/Goat IgG:DyLight®488
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader
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Description Donkey anti Sheep/Goat IgG:HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
Native Bovine Ubiquitin 9400-1502 E 25 mg loader
List Price Your Price
loader
Description Native Bovine Ubiquitin

RRID
AB_1720133
UniProt
P62990
View more products with UBIQUITIN specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Bovine Products